epsL

epsL
168

phosphoglycosyl transferase, binds N,N′-diacetylbacillosamine to the lipid acceptor undecaprenol phosphate (UndP), extracellular polysaccharide synthesis

Locus
BSU_34250
Molecular weight
22.93 kDa
Isoelectric point
9.97
Protein length
202 aaSequenceBlast
Gene length
609 bpSequenceBlast
Function
biofilm formation
Product
phosphoglycosyl transferase
Essential
no
Synonyms
epsL, yvfC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG2148 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, AbrB regulon, SinR regulon, RemA regulon, EAR riboswitch

Gene
Coordinates
3,516,880 → 3,517,488
The protein
Catalyzed reaction/ biological activity
UDP-N,N’-diacetylbacillosamine + undecaprenyl-phosphate (UndP) --> undecaprenyl-PP-diNAcBac PubMed
Protein family
Bacterial sugar transferase family (with TuaA, according to UniProt)
Structure
5W7L (PDB) (from Campylobacter concisus, 58% identity) PubMed
P71062 (AlphaFold)
Paralogous protein(s)
TuaA/1, TuaA/2
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO
Description
PubMed
Regulation
repressed by SinR PubMed
Regulatory mechanism
RemA: activation, PubMed, in remA regulon
AbrB: repression, PubMed, in abrB regulon
SinR: anti-activation, (prevents binding of RemA) PubMed, in sinR regulon
EAR riboswitch: processive antitermination, in EAR riboswitch
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
induction by sequestration of SinR by SinI or SlrA PubMed
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epsA→epsO

2025-04-02 11:08:39

ghost

150

972f8e32dbf02ccc05ec1b86b52f3e7c715bf4da

DAC70A15EA1F43CB18727990D26DF72F59759165

Biological materials
Mutant
MGNA-A065 (yvfC::erm), available at the NBRP B. subtilis, Japan
BKE34250 (ΔepsL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAGATCAAAAAGTCGTTTCA,  downstream forward: _UP4_ACCGGAAGCGGAGATGTGTC
BKK34250 (ΔepsL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAGATCAAAAAGTCGTTTCA,  downstream forward: _UP4_ACCGGAAGCGGAGATGTGTC
Labs working on this gene/protein
Richard Losick, Harvard Univ., Cambridge, USA homepage
References
Reviews
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Research papers
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The EAR RNA switch
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D67665412B78B56752B460219445E4BEF52C3A1A

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Time of last update: 2025-04-08 03:51:17

Author of last update: Jstuelk