epsD
168
UDP-GlcNAc-dependent N-acetyl glucosamine transferase , extracellular polysaccharide synthesis
locus
BSU_34340
Molecular weight
43.41 kDa
pI
8.73
function
[wiki|biofilm formation]
product
UDP-GlcNAc-dependent N-acetyl glucosamine transferase
essential
no
synonyms
epsD, yveN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0438 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,525,250 3,526,395
Phenotypes of a mutant
smooth colonies on MsGG medium, no [wiki|biofilm formation] [Pubmed|22113911]
The protein
Catalyzed reaction/ biological activity
undecaprenyl-PP-diNAcBac + N-acetyl-glucosamine --> undecaprenyl-PP-diNAcBac-NAc-glucosamine + [metabolite|UDP] [pubmed|37650625]
Protein family
[wiki|glycosyltransferase 1 family] (according to UniProt)
[wiki|Glycosyltransferase 4 subfamily] (according to UniProt)
Structure
[PDB|2JJM] (from B. anthracis, 24% identity) [pubmed|18712829]
[AF|P71053]
Expression and Regulation
Operons
genes
[gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]-[gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]-[gene|5DB168A3D087AAEB003D7548A1A4356ABD07F712|epsC]-[gene|223E979276F07D69D0DDAEE906023A8AB4F37F8E|epsD]-[gene|5D717F9F54693FD0AA8BC49E10348637858F88B9|epsE]-[gene|DB47BA2A5E3BED51DD8630E7D321C099D74B46CF|epsF]-[gene|C4D81976AAE888C4FE89A2EE5CF9A763CAD645FF|epsG]-[gene|BD843389F95BFB905071547AB243413A617F12A8|epsH]-[gene|6EBD8F7065B6C1996067F3120DD9CFEA91D3A444|epsI]-[gene|5272E807B6B98541EA155AA9748320E25767F096|epsJ]-[gene|66076952E512D53FC1FC62455A86ACFA21AE120D|epsK]-[gene|D67665412B78B56752B460219445E4BEF52C3A1A|epsL]-[gene|40EA584CF77FFF7D7E4E74B414EC69ADE3A1585B|epsM]-[gene|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN]-[gene|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO]
description
[Pubmed|15661000]
regulation
repressed by [protein|search|SinR] [Pubmed|15661000]
regulatory mechanism
[protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]: activation, [Pubmed|23646920], in [regulon|protein:6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]: anti-activation, (prevents binding of [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]) [Pubmed|23646920], in [regulon|protein:5A6FBAE6553343092862CB79E150F934978C32A9|sinR regulon]
EAR riboswitch: processive antitermination, in [regulon|other_regulator:EAR riboswitch|EAR riboswitch]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|15661000], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
induction by sequestration of [protein|search|SinR] by [protein|search|SinI] or [protein|search|SlrA] [PubMed|15661000,19788541]
Biological materials
Mutant
MGNA-A070 (yveN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/70 NBRP B. subtilis, Japan]
BKE34340 ([gene|223E979276F07D69D0DDAEE906023A8AB4F37F8E|epsD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCACTCCCCCTAATG, downstream forward: _UP4_GTATGAACTCAGGACCGAAA
BKK34340 ([gene|223E979276F07D69D0DDAEE906023A8AB4F37F8E|epsD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCACTCCCCCTAATG, downstream forward: _UP4_GTATGAACTCAGGACCGAAA
labs
[wiki|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]
References
Reviews
Research papers
The EAR [wiki|RNA switch]
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Time of last update: 2024-12-22 08:38:11
Author of last update: Jstuelk