ywjH
168
transaldolase
locus
BSU_37110
Molecular weight
22.77 kDa
pI
5.88
function
pentose phosphate pathway
product
transaldolase
essential
no
ec
2.2.1.2
synonyms
ywjH, tal
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0176 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,807,754 3,808,392
The protein
Catalyzed reaction/ biological activity
D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate --> -D-fructose 6-phosphate + D-erythrose 4-phosphate (according to UniProt)
Protein family
transaldolase family (single member, according to UniProt)
Structure
[PDB|3R8R] [Pubmed|22212631]
[AF|P19669]
Modification
phosphorylation on Ser-39 [Pubmed|17218307,16493705,17726680], ''in vitro'' phosphorylated by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] [Pubmed|20389117]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]-[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]
description
[Pubmed|11489127]
regulation
constitutively expressed [Pubmed|11489127]
Biological materials
Mutant
MGNA-B264 (ywjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1263 NBRP B. subtilis, Japan]
BKE37110 ([gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCAAAAAGCCTCCCT, downstream forward: _UP4_TAATGAAAGGGGCGGCAAAC
BKK37110 ([gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCAAAAAGCCTCCCT, downstream forward: _UP4_TAATGAAAGGGGCGGCAAAC
Expression vectors
for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172]: pGP819, available in [wiki|Jrg Stlke]'s lab, [pubmed|20389117]
pGP1790 (expression of ''[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]'' in ''B. subtilis'', in [wiki|pGP1389]), available in [wiki|Jrg Stlke]'s lab
GP1409 (''ywjH''-''Strep'' ''(spc)'') & GP1411 (''ywjH''-''Strep'' ''(cat)''), purification from ''B. subtilis'', for [wiki|SPINE], available in [wiki|Jrg Stlke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jrg Stlke]'s lab
FLAG-tag construct
GP1407 (spc, based on [wiki|pGP1331]), available in [wiki|Jrg Stlke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-10-14 12:09:52
Author of last update: Jstuelk