fbaA
168
[metabolite|fructose-1,6-bisphosphate] aldolase, glycolytic/ gluconeogenic enzyme
locus
BSU_37120
Molecular weight
30.25 kDa
pI
5.03
function
enzyme in glycolysis/ gluconeogenesis
product
[metabolite|fructose-1,6-bisphosphate] aldolase
essential
no
ec
4.1.2.13
synonyms
fbaA, fba, fba1, tsr
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0191 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,808,512 3,809,369
Phenotypes of a mutant
essential [Pubmed|12682299], non-essential according to [Pubmed|23420519]
The protein
Catalyzed reaction/ biological activity
D-[metabolite|fructose-1,6-bisphosphate] --> D-[metabolite|glyceraldehyde-3-P] + [metabolite|dihydroxyacetone-P] (according to UniProt)
Protein family
class II fructose-bisphosphate aldolase family (with [protein|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|iolJ], according to UniProt)
[wiki|Domains]
2 x [metabolite|dihydroxyacetone-P] binding domain (210212), (231234)
[wiki|Cofactors]
Zn2+ (Metalloenzyme)
Structure
[PDB|3Q94] (from ''Bacillus anthracis'')
[AF|P13243]
Modification
phosphorylation on Thr-212 and Thr-234 [Pubmed|17218307]
Effectors of protein activity
Inhibited by [metabolite|2-oxoglutarate], [metabolite|oxaloacetate] and [metabolite|pyruvate] [Pubmed|24624] [Pubmed|15125960]
Activated by NH4+ [Pubmed|15125960]
Paralogous protein(s)
[protein|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|iolJ]
Additional information
Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution
extensive information on the structure and enzymatic properties of FbaA can be found at [http://www.proteopedia.org/wiki/index.php/Fructose_Bisphosphate_Aldolase Proteopedia]
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]
description
[Pubmed|11489127]
regulation
constitutively expressed [Pubmed|11489127]
genes
[gene|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]-[gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]-[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]-[gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]-[gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]-[gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]-[gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]
description
[pubmed|39427753]
expression
readthrough-transcription from the [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]-dependent promoter after the initiation of sporulation in the mother cell [pubmed|39427753]
genes
[gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]-[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]
description
[Pubmed|11489127]
regulation
constitutively expressed [Pubmed|11489127]
Biological materials
Mutant
GP591 (''fbaA''::''cat''), available in [wiki|Jrg Stlke]'s lab, [Pubmed|23420519]
GP596 (''fbaA''::''erm''), available in [wiki|Jrg Stlke]'s lab, [Pubmed|23420519]
BKE37120 ([gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGAATGTCCTCCTTA, downstream forward: _UP4_TAATTCAATTGGAACTTTTT
BKK37120 ([gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGAATGTCCTCCTTA, downstream forward: _UP4_TAATTCAATTGGAACTTTTT
Expression vectors
for expression in ''B. subtilis'', in [wiki|pBQ200]: pGP1423, available in [wiki|Jrg Stlke]'s lab
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP88, available in [wiki|Jrg Stlke]'s lab, [pubmed|19193632]
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP395, available in [wiki|Jrg Stlke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jrg Stlke]'s lab [pubmed|19193632]
lacZ fusion
pGP601 (in [wiki|pAC6]), available in [wiki|Jrg Stlke]'s lab [pubmed|11489127]
References
Reviews
Original Publications
Page visits: 7321
Time of last update: 2024-12-23 23:35:09
Author of last update: Jstuelk