rho
168
transcriptional termination protein, prevents the formation of antiterminator-like RNA structures that could otherwise compete with the terminator hairpin, involved in balanced regulation of cell motility, [category|SW.4.1.2|Biofilm formation], and [category|SW.4.2|Sporulation]
locus
BSU_37080
Molecular weight
48.51 kDa
pI
6.86
function
transcriptional termination
product
transcriptional termination protein
essential
no
synonyms
rho
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1158 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,803,400 3,804,683
Phenotypes of a mutant
loss of motility due to poor expression of the [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD] regulon, this can be partially reversed by inactivation of [gene|920F91E748EE079FF864011D9052B073567C41E4|slrR] [pubmed|28723971]
no [category|SW.4.1.2|Biofilm formation], this can be suppressed by deletion of [gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] and by switching of expression of the [gene|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO]-[gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA] antisense transcript [pubmed|28723971]
increased efficiency of [category|SW.4.2|Sporulation], due to enhanced [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A] phosphorylation [pubmed|28723971]
defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]
The protein
Catalyzed reaction/ biological activity
prevents the formation of antiterminator-like RNA structures that could otherwise compete with the terminator hairpin [pubmed|36154805]
Protein family
Rho family (single member, according to UniProt)
[wiki|Domains]
[wiki|CSD domain] (aa 54-127) (according to the InterPro database)
Rho RNA-BD domain (aa 51-125) (according to UniProt)
Structure
[PDB|1PVO] (Rho from ''E. coli'') [Pubmed|12859904]
[PDB|8HSR] ([protein|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]-[wiki|RNA polymerase] termination complex from Thermus thermophilus) [pubmed|36753546]
[AF|Q03222]
Expression and Regulation
Operons
genes
[gene|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]-[gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]-[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]-[gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]-[gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]-[gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]-[gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]
description
[pubmed|39427753]
expression
readthrough-transcription from the [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]-dependent promoter after the initiation of sporulation in the mother cell [pubmed|39427753]
genes
[gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]-[gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]
description
[pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, during vegetative growth [pubmed|39427753], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, after initiation of sporulation in the forespore [pubmed|39427753], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Biological materials
Mutant
GP2673 ([gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]::''ermC''), available in [wiki|Jörg Stülke]'s lab
BKE37080 ([gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACACCACGCTTTT, downstream forward: _UP4_TAAATTGTATTTGCAAAAGA
BKK37080 ([gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACACCACGCTTTT, downstream forward: _UP4_TAAATTGTATTTGCAAAAGA
References
Reviews
Original Publications
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Time of last update: 2024-12-18 23:34:28
Author of last update: Jstuelk