glpX
168
class II fructose-1,6-bisphosphatase, gluconeogenesis
locus
BSU_37090
Molecular weight
33.80 kDa
pI
5.07
function
gluconeogenesis
product
class II fructose-1,6-bisphosphatase
essential
no
ec
3.1.3.11
synonyms
glpX, ywjI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1494 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,805,090 3,806,055
The protein
Catalyzed reaction/ biological activity
β-D-fructose 1,6-bisphosphate + H2O --> β-D-fructose 6-phosphate + phosphate (according to UniProt)
Protein family
FBPase class 2 family (single member, according to UniProt)
Structure
[PDB|3D1R] (from ''E. coli'', 49% identity, 68% similarity)[Pubmed|19073594]
[AF|Q03224]
Kinetic information
K(M) for FBP: 20 M [Pubmed|19270101]
Expression and Regulation
Operons
genes
[gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]-[gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]
description
[Pubmed|19270101]
regulation
constitutive [Pubmed|19270101]
genes
[gene|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]-[gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]-[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]-[gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]-[gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]-[gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]-[gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]
description
[pubmed|39427753]
expression
readthrough-transcription from the [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]-dependent promoter after the initiation of sporulation in the mother cell [pubmed|39427753]
Biological materials
Mutant
MGNA-B658 (ywjI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1657 NBRP B. subtilis, Japan]
BKE37090 ([gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCAACACGATCTCCTC, downstream forward: _UP4_TAACAAACAGAAAAAGCGGG
BKK37090 ([gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCAACACGATCTCCTC, downstream forward: _UP4_TAACAAACAGAAAAAGCGGG
References
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Time of last update: 2024-12-24 01:43:25
Author of last update: Melvin.boenninger