sigH
168
[wiki|RNA polymerase] [wiki|sigma factor] SigH, not fully active in laboratory strains due to a mutation (V117A)
locus
BSU_00980
Molecular weight
25.30 kDa
pI
5.15
function
transcription of early stationary phase genes ([wiki|sporulation], competence)
product
[wiki|RNA polymerase] [wiki|sigma factor] SigH
essential
no
synonyms
sigH, spo0H
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1595 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
116,600 → 117,256
The protein
Protein family
[wiki|Sigma-70 factor family] (according to UniProt)
[wiki|Domains]
[wiki|HTH luxR-type domain] (aa 179-206) (according to InterPro)
Structure
[PDB|3MZY] (from Fusobacterium nucleatum, 40% identity)
[AF|P17869]
Additional information
not fully active in laboratory strains due to a mutation (V117A) [pubmed|27501195]
the poor interaction of [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH] with the [wiki|RNA polymerase] is responsible for the poor [wiki|sporulation] at high salinity, this phenotype can be partially suppressed by specific mutations in [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH] [Pubmed|26712348]
Expression and Regulation
Operons
genes
[gene|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]-[gene|BBD82B93D5E880AB7093ED53DDB149B0E0B3BAE4|rpmGB]-[gene|1007EA15FB7AF81A44400287DB68BCC497158ACF|secE]
description
[Pubmed|22383849]
regulation
repressed during logrithmic growth ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]) [Pubmed|7592498]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|7592498], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1898930], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE00980 (Δ[gene|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCCGATCCCCCCGGCGC, downstream forward: _UP4_TAATAGGAATTTATGCTATA
BKK00980 (Δ[gene|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCCGATCCCCCCGGCGC, downstream forward: _UP4_TAATAGGAATTTATGCTATA
labs
[wiki|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
References
Reviews
Original Publications
The [wiki|SigH regulon]
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Time of last update: 2024-12-24 04:15:54
Author of last update: Jstuelk