murAB
168
UDP-N-acetylglucosamine 1-carboxyvinyltransferase, required for efficient spore engulfment
locus
BSU_37100
Molecular weight
45.85 kDa
pI
5.83
function
peptidoglycan precursor biosynthesis
product
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
essential
no
ec
2.5.1.7
synonyms
murAB, murZ, lssF, rev-4
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0766 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,806,086 3,807,375
The protein
Catalyzed reaction/ biological activity
phosphoenolpyruvate + UDP-N-acetyl-α-D-glucosamine --> phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-α-D-glucosamine(according to UniProt)
Protein family
EPSP synthase family (with [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA] and [protein|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE], according to UniProt)
Structure
[PDB|3SG1] (from ''B. anthracis'', 50% identity, 81% similarity)
[AF|P19670]
Paralogous protein(s)
[protein|DA763F7C2192655C590752C2A285ADF902B11BCA|murAA]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]-[gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]
description
[Pubmed|19270101]
regulation
constitutive [Pubmed|19270101]
genes
[gene|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]-[gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]-[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]-[gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]-[gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]-[gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]-[gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]
description
[pubmed|39427753]
expression
readthrough-transcription from the [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]-dependent promoter after the initiation of sporulation in the mother cell [pubmed|39427753]
Biological materials
Mutant
BKE37100 ([gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAGTCTCCTTCCATA, downstream forward: _UP4_TAATGGTTTCTTGTGAGAGG
BKK37100 ([gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAGTCTCCTTCCATA, downstream forward: _UP4_TAATGGTTTCTTGTGAGAGG
Expression vectors
pGP2595: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-23 21:09:51
Author of last update: Jstuelk