bioA

bioA
168

lysine-8-amino-7-oxononanoate aminotransferase

Locus
BSU_30230
Molecular weight
49.95 kDa
Isoelectric point
5.32
Protein length
448 aaSequenceBlast
Gene length
1344 bpSequenceBlast
Function
biosynthesis of biotin
Product
lysine-8-amino-7-oxononanoate aminotransferase
Essential
no
E.C.
2.6.1.62
Synonyms
bioA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0161 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,093,343 → 3,094,689
The protein
Catalyzed reaction/ biological activity
8-amino-7-oxononanoate + L-lysine --> (S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate (according to UniProt)
Protein family
Class-III pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)
Cofactors
PLP (according to UniProt)
Structure
3DRD (PDB)
P53555 (AlphaFold)
Paralogous protein(s)
YhxA, (33,6%)
Expression and Regulation
Operons
Genes
bioW-bioA-bioF-bioD-bioB-bioI-ytbQ
Description
PubMed
Regulation
repressed in the presence of biotin (BirA) PubMed
Regulatory mechanism
BirA: repression, PubMed, in birA regulon
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bioW→ytbQ

2025-04-03 13:40:07

ghost

82

8e74711c48d33750c45114afa6e28dd08cdf4ea5

F7255B320407AE19B7B38F8E863B5EF4A9BF3D52

Biological materials
Mutant
GP4753 (trpC2 ΔbioA::neo), available in Jörg Stülke's lab
BKE30230 (ΔbioA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCATGAGTCATGATCTTC,  downstream forward: _UP4_AGCCTTGAAGATTGATTCCT
BKK30230 (ΔbioA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCATGAGTCATGATCTTC,  downstream forward: _UP4_AGCCTTGAAGATTGATTCCT
References
Reviews
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Original Publications
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68C86FED3575B864C576DFB73FE7B5BF83994D1F

Page visits: 3415

Time of last update: 2025-04-07 13:59:43

Author of last update: Robert.warneke