bioF
168
8-amino-7-oxononanoate synthase
locus
BSU_30220
Molecular weight
42.42 kDa
pI
6.28
function
biosynthesis of biotin
product
8-amino-7-oxononanoate synthase
essential
no
ec
2.3.1.47
synonyms
bioF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0156 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,092,184 3,093,353
The protein
Catalyzed reaction/ biological activity
pimeloyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO2 [pubmed|29054876]
Protein family
[wiki|class-II pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
PLP (according to UniProt)
Structure
[PDB|3A2B] (Serine palmitoyltransferase from Sphingobacterium multivorum, 37% identity) [pubmed|19564159]
[AF|P53556]
Paralogous protein(s)
[protein|1BC05699DEB3A16944FB8305F22976AED75805E8|kbl]
Expression and Regulation
Operons
genes
[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]
description
[Pubmed|8763940,8892842]
regulation
repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|8763940,8892842]
regulatory mechanism
[protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]: repression, [Pubmed|8763940,8892842], in [regulon|protein:F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA regulon]
Biological materials
Mutant
GP4750 (trpC2 Δ[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]::neo), available in [wiki|Jörg Stülke]'s lab
BKE30220 ([gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCTAACCGCTCGTTTAACC, downstream forward: _UP4_ATCGGAAAGGAGCTGCACAT
BKK30220 ([gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCTAACCGCTCGTTTAACC, downstream forward: _UP4_ATCGGAAAGGAGCTGCACAT
References
Reviews
Original Publications
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Time of last update: 2025-04-03 12:58:21
Author of last update: Robert.warneke