bioW
168
pimeloyl-CoA synthase (6-carboxyhexanoate-CoA ligase)
locus
BSU_30240
Molecular weight
29.48 kDa
pI
5.55
function
biosynthesis of biotin
product
pimeloyl-CoA synthase
essential
no
ec
6.2.1.14
synonyms
bioW
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1424 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,094,679 → 3,095,455
Phenotypes of a mutant
requires biotin for growth [Pubmed|28196402]
The protein
Catalyzed reaction/ biological activity
ATP + CoA + heptanedioate --> AMP + diphosphate + heptanedioyl-CoA (according to UniProt)
Protein family
bioW family (single member, according to UniProt)
Structure
[PDB|5FLG] [pubmed|28414710]
[AF|P53559]
Expression and Regulation
Operons
genes
[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]
description
[Pubmed|8763940,8892842]
regulation
repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|8763940,8892842]
regulatory mechanism
[protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]: repression, [Pubmed|8763940,8892842], in [regulon|protein:F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA regulon]
Biological materials
Mutant
GP4743 (trpC2 Δ[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]::neo), available in [wiki|Jörg Stülke]'s lab
BKE30240 (Δ[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTTCTCGCCCTTTCA, downstream forward: _UP4_ATTTTAATAGAGTGGGAGGA
BKK30240 (Δ[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTTCTCGCCCTTTCA, downstream forward: _UP4_ATTTTAATAGAGTGGGAGGA
lacZ fusion
pBP627 (in [wiki|pAC7]), available in [wiki|Fabian Commichau]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-11-15 13:53:53
Author of last update: Robert.warneke