bioB
168
biotin synthase
locus
BSU_30200
Molecular weight
36.77 kDa
pI
5.55
function
biosynthesis of biotin
product
biotin synthase
essential
no
ec
2.8.1.6
synonyms
bioB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0502 (Galperin et al., 2021)
This gene is a member of the following regulons
The protein
Catalyzed reaction/ biological activity
[sulfur carrier]-SH + dethiobiotin + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)
Protein family
[wiki|Radical SAM superfamily] (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|1R30] (from ''E. coli'', 36% identity) [Pubmed|14704425]
[AF|P53557]
Expression and Regulation
Operons
genes
[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]
description
[Pubmed|8763940,8892842]
regulation
repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|8763940,8892842]
regulatory mechanism
[protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]: repression, [Pubmed|8763940,8892842], in [regulon|protein:F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA regulon]
Biological materials
Mutant
GP4752 (trpC2 Δ[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]::neo), available in [wiki|Jörg Stülke]'s lab
GP4764 (trpC2 Δ[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]::lox72), available in [wiki|Jörg Stülke]'s lab
BKE30200 ([gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGTTCCATCCATTGATTCA, downstream forward: _UP4_TGAAAGAATCAATAAAAGCA
BKK30200 ([gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGTTCCATCCATTGATTCA, downstream forward: _UP4_TGAAAGAATCAATAAAAGCA
References
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Time of last update: 2025-04-01 04:30:06
Author of last update: Jstuelk