recU
168

Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates RecA activities, required for efficient survival and replication restart after replication-transcription conflicts

Locus

BSU_22310

Molecular weight

23.81 kDa

Isoelectric point

9.51

Protein length

206 aaSequenceBlast

Gene length

621 bpSequenceBlast

Function

DNA repair/ recombination and chromosome segregation

Product

Holliday junction resolvase

Essential

Synonyms

recU, prfA, yppB, jopB

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3331 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

2,340,802 → 2,341,422

Phenotypes of a mutant

a recU recG double mutant is not viable PubMed

a recU recJ double mutant is not viable PubMed

the inactivations of ''recU and ruvA-ruvB'' are synthetically lethal PubMed

loss of plasmid transformation, this can be suppressed by deletion of recA PubMed

reduced survival after DNA replication arrest imposed by inhibition of PolC activity PubMed

The protein

Catalyzed reaction/ biological activity

binds Holliday junctions with high affinity PubMed

inhibits RecA filament growth, facilitates RecA disassembly from preformed filaments PubMed

endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction) (according to UniProt)

promotes a net RecA disassembly from viral ssDNAs not homologous to the host genome PubMed

Protein family

RecU family (single member, according to UniProt)

Structure

1ZP7 (PDB) PubMed

P39792 (AlphaFold)

Effectors of protein activity

RecU-mediated Holliday junction resolution is stimulated by RuvB-ATP PubMed

DisA PubMed

Paralogous protein(s)

Mfd

Interactions