recJ
168
single-strand DNA-specific exonuclease, acts together with [protein|E6EA8098E37A4A2F7370920AC1A500EE4319AAA2|recQ] in replication fork maintenance, crucial for the [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-dependent acquisition of homologous genes from related species by natural transformation
locus
BSU_27620
Molecular weight
87.83 kDa
pI
5.18
function
replication fork maintenance
product
single-strand DNA-specific exonuclease
essential
no
synonyms
recJ, yrvE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4199 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,823,419 → 2,825,779
Phenotypes of a mutant
a [gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ] double mutant is not viable [pubmed|16020779]
''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]
suppression of lethality of [gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA] inactivation [pubmed|32793628]
improved survival after [category|SW.3.1.1|DNA replication] arrest imposed by inhibition of [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC] activity [pubmed|36574412]
The protein
Protein family
RecJ family (with [protein|CDFA18E1C0445302ED4D8F93F88822EC6BD646BB|yorK], according to UniProt)
[wiki|Domains]
[wiki|DHH-DHHA1 domain]
[wiki|Cofactors]
Mn2+ [pubmed|27058167]
Structure
[PDB|5F54] (from Deinococcus radiodurans, the [wiki|DHH-DHHA1 domain], 32% identity) [pubmed|27058167]
[AF|O32044]
Paralogous protein(s)
[protein|CDFA18E1C0445302ED4D8F93F88822EC6BD646BB|yorK]
[wiki|Localization]
associated with the [wiki|replisome] [pubmed|21170359]
bound to replication forks, but also assembles at many sites on the nucleoid upon DNA damage induction [pubmed|30401797]
Expression and Regulation
Operons
genes
[gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]-[gene|38DF74800FC5D54183C58B0EB82BD9A3EB93EE33|apt]
description
[Pubmed|15241682]
regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
additional information
[protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]
Biological materials
Mutant
GP895 ([gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]::''kan''), available in [wiki|Jörg Stülke]'s lab [pubmed|22178973]
MGNA-B516 (yrvE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1515 NBRP B. subtilis, Japan]
BKE27620 (''[gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]''::''erm'', available in the BGSC, in [wiki|Fabian Commichau]'s, and in [wiki|Jörg Stülke]'s labs) [pubmed|28189581]
BKE27620 (Δ[gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATTCACCCCTCAACC, downstream forward: _UP4_AGTACGAGGAGGACATAAAA
BKK27620 (Δ[gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATTCACCCCTCAACC, downstream forward: _UP4_AGTACGAGGAGGACATAAAA
References
Reviews
Original Publications
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Time of last update: 2025-04-03 09:37:24
Author of last update: Jstuelk