recU
168
Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] activities, required for efficient survival and replication restart after replication-transcription conflicts
locus
BSU_22310
Molecular weight
23.81 kDa
pI
9.51
function
[wiki|DNA repair/ recombination] and [wiki|chromosome segregation]
product
Holliday junction resolvase
essential
no
synonyms
recU, prfA, yppB, jopB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3331 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,340,802 → 2,341,422
Phenotypes of a mutant
a [gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] [gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG] double mutant is not viable [pubmed|16020779]
a [gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ] double mutant is not viable [pubmed|16020779]
the inactivations of ''[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]'' and ''[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]'' are synthetically lethal [Pubmed|16020779,24770420]
loss of plasmid transformation, this can be suppressed by deletion of [gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] [pubmed|30050509]
reduced survival after [category|SW.3.1.1|DNA replication] arrest imposed by inhibition of [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC] activity [pubmed|36574412]
The protein
Catalyzed reaction/ biological activity
binds Holliday junctions with high affinity [Pubmed|24770420]
inhibits [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] filament growth, facilitates [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] disassembly from preformed filaments [pubmed|30050509]
endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction) (according to UniProt)
promotes a net [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] disassembly from viral ssDNAs not homologous to the host genome [pubmed|31876108]
Protein family
RecU family (single member, according to UniProt)
Structure
[PDB|1ZP7] [Pubmed|16154091]
[AF|P39792]
Effectors of protein activity
[protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]-mediated Holliday junction resolution is stimulated by [protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-ATP [Pubmed|24770420]
[protein|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA] [pubmed|34768753]
Paralogous protein(s)
[protein|AD1F39823ABCE2A222259B879513752942C59590|mfd]
[wiki|Interactions]
part of the [protein|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] resolvasome [pubmed|24770420]
Expression and Regulation
Operons
genes
[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]-[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]
description
[Pubmed|7814321]
regulation
constitutive during vegetative growth [Pubmed|15758244]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22211522], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP891 (''recU''::''cat''), available in [wiki|Jörg Stülke]'s lab [pubmed|22178973]
BKE22310 ( ''recU''::''ermC''), (available at the [http://bgsc.org/ BGSC] and in [wiki|Fabian Commichau]'s lab) [pubmed|28189581]
BP784 ( ''recU''::''ermC''), (available in [wiki|Fabian Commichau]'s lab)
1A895 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A895&Search=1A895 BGSC]
BKE22310 (Δ[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCATCCTCCTTAT, downstream forward: _UP4_TGATTAACGAAAGGTTGAGA
BKK22310 (Δ[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCATCCTCCTTAT, downstream forward: _UP4_TGATTAACGAAAGGTTGAGA
References
Reviews
Original Publications
RecU in other organisms
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Time of last update: 2024-12-26 10:45:34
Author of last update: Jstuelk