ruvA
168
Holliday junction DNA helicase, part of the [protein|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB] branch migration translocase and the [protein|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] resolvasome
locus
BSU_27740
Molecular weight
22.44 kDa
pI
5.38
function
[wiki|DNA repair/ recombination]
product
Holliday junction DNA helicase
essential
no
synonyms
ruvA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0632 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,836,165 2,836,770
Phenotypes of a mutant
a [gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB] [gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG] mutant is not viable [pubmed|16020779]
the inactivations of ''[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]'' and ''[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]'' are synthetically lethal [Pubmed|24770420]
the inactivations of ''[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]'' and ''[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]'' are synthetically lethal [pubmed|28527403]
The protein
Catalyzed reaction/ biological activity
inhibits resolution of Holliday junctions by [protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB] [Pubmed|24770420]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
ruvA family (single member, according to UniProt)
Structure
[PDB|1BDX] (from E. coli, 34% identity) [pubmed|9628481]
[AF|O05392]
Effectors of protein activity
[protein|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA] [pubmed|34768753]
[wiki|Interactions]
part of the [protein|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] resolvasome [pubmed|24770420]
Expression and Regulation
Operons
genes
[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[gene|6CDF1836F5B613A6718308A4A27D713193EA197B|yrzS]-[gene|23174B2A19DEDF2F20858A005D0681D36E15E6E3|queA]-[gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt]-[gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF]
description
[pubmed|22383849]
regulation
the mRNA is processed between [gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt] and [gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
genes
[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]
description
[Pubmed|16267290]
regulation
induced upon DNA damage ([protein|search|LexA]) [Pubmed|16267290]
regulatory mechanism
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
Biological materials
Mutant
GP3530 (Δ([gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB])::kan), available in [wiki|Jörg Stülke]'s lab
1A896 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A896&Search=1A896 BGSC]
BKE27740 ([gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGCAAATTCACCTCTTTG, downstream forward: _UP4_AAACTATTAAAGTAAGGTGA
BKK27740 ([gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27740 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CACGCAAATTCACCTCTTTG, downstream forward: _UP4_AAACTATTAAAGTAAGGTGA
References
Reviews
Original Publications
Page visits: 3358
Time of last update: 2025-02-01 18:43:39
Author of last update: Jstuelk