citG
168
fumarase
locus
BSU_33040
Molecular weight
50.36 kDa
pI
5.47
function
TCA cycle
product
fumarate hydratase
essential
no
ec
4.2.1.2
synonyms
citG, fumC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0114 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,389,024 3,390,412
Phenotypes of a mutant
arginine is toxic for the mutant, this can be bypassed by inactivation of [gene|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC] encoding the major arginine transporter [pubmed|38483038]
The protein
Catalyzed reaction/ biological activity
(S)-malate --> fumarate + H2O (according to UniProt)
Protein family
class-II fumarase/aspartase family (with [protein|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB], according to UniProt)
Structure
[PDB|1FUO] (from ''E. coli'', 64% identity) [Pubmed|8909293]
[AF|P07343]
Paralogous protein(s)
[protein|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]
[wiki|Localization]
forms discrete foci in the cytoplasm [pubmed|29140245]
colocalizes with DNA upon DNA damage (MMS treatment) [pubmed|29140245]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG]
description
[Pubmed|3923430]
regulation
constitutive
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|3130545,2509422,2509423], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|3130545,2509422], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
genes
[gene|B66AE23754A9CD4D5BCFC75CE6918522C7A22DA6|yvzF]-[gene|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG]
description
[Pubmed|22383849]
Biological materials
Mutant
GP718 (spec), available in [wiki|Jrg Stlke]'s lab [pubmed|20933603]
GP2340 ''[gene|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG]''::''kan'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [wiki|Jrg Stlke]'s lab
GP2341 ''[gene|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG]''::''lox72'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [wiki|Jrg Stlke]'s lab
BKE33040 ([gene|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATGTATCCCTCCATA, downstream forward: _UP4_TAATAGGAAGAACGGCTGCT
BKK33040 ([gene|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATGTATCCCTCCATA, downstream forward: _UP4_TAATAGGAAGAACGGCTGCT
Expression vectors
pGP1122 (N-terminal Strep-tag, for [wiki|SPINE], purification from ''B. subtilis'', in [wiki|pGP380]) (available in [wiki|Jrg Stlke]'s lab) [pubmed|20933603]
pGP3706: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jrg Stlke]'s lab
FLAG-tag construct
GP1132 (spc, based on [wiki|pGP1331]), available in [wiki|Jrg Stlke]'s lab
lacZ fusion
pGP387 (in [wiki|pAC7]), available in [wiki|Jrg Stlke]'s lab
Antibody
**
GFP fusion
GP1433 (spc, based on [wiki|pGP1870]), available in the [wiki|Jrg Stlke] lab
References
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Time of last update: 2024-12-22 08:19:16
Author of last update: Jstuelk