rocC

rocC
168

amino acid permease, major transporter for arginine

locus
BSU_37760
Molecular weight
51.56 kDa
pI
8.41
Protein length
Gene length
function
arginine, ornithine and citrulline uptake
product
major transporter for arginine
essential
no
synonyms
rocC, ipa-78d

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0833 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,875,780  3,877,192
The protein
Catalyzed reaction/ biological activity
uptake of arginine [pubmed|38483038]
Protein family
[wiki|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)
Structure
[PDB|6F34] (from Geobacillus kaustophilus, 24% identity) [pubmed|29416041]
[AF|P39636]
Paralogous protein(s)
[protein|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE], [protein|B6A920650919D5BBBD75CCA58C30A0D8ECE64DA3|ybgF], [protein|C7FEA1D9C7C96C39CEE83CE3F13D412DE6AFE10E|ydgF], [protein|CE4A49F5CCC2D36C5CE5AD7AE7F0A70F2D4F425C|aapA], [protein|1594BA83CED2DA9F86C1CE2A0DC6E1C9B665EACB|gabP], [protein|219586A3F378DC38EA076FD39B99D41136BDE722|alaP], [protein|423AEC9D260BE53AD22851D999244B89BEB3E84D|hutM], [protein|8C4A8961B2514A8AD531AFB104B10C0797D02AC2|yvbW], [protein|9AD0E43119BDC65D5F67CE55CD9417E1E6BAE058|aimB]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA]-[gene|D8D79BC7E98754963CA9DC577B90929A5A9CE051|rocB]-[gene|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC]
description
[Pubmed|8113162]
regulation
expression during spore [wiki|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
induced in the presence of arginine ([protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]) [Pubmed|7565595]
induced in the presence of arginine, citrulline or ornithine (with ornithine as the molecular inducer) ([protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]) [pubmed|37343703]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]: activation, [Pubmed|8113162], in [regulon|protein:BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR regulon]
[protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]: activation, [Pubmed|7565595], in [regulon|protein:62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [Pubmed|8113162], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
additional information
expression of the [gene|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA]-[gene|D8D79BC7E98754963CA9DC577B90929A5A9CE051|rocB]-[gene|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC] operon is increased upon depletion of [gene|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA] (resulting from increased [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] expression) [http://www.nature.com/articles/nmicrobiol20157 Reference]
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[gene|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA]→[gene|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC]

2024-10-03 20:58:41

Jstuelk

119

2DC790ED3D8C4B63BB982C1F1D977B49CCE4042B

BCA34B2E2917A99583D79922F2E2CBC45D25A78E

Biological materials
Mutant
GP4151 (Δ[gene|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC]::''aphA3''), available in [wiki|Jörg Stülke]'s lab
GP4156 (Δ[gene|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC]::''aphA3'', Δ[gene|8D9529D46E11D8B2826A98642706ADCBE1E3434A|artP]-[gene|62BD291B91DB0358754957D84A1F3636C247A697|artQ]-[gene|6882EF93EC82872B25C88104F62D6603D2514890|artR]::''ermC'', Δ[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]::''tet'',), available in [wiki|Jörg Stülke]'s lab
BKE37760 ([gene|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTCATTTTTATGATTCT,  downstream forward: _UP4_TAGAAAAAGCTCTCCGGGAG
BKK37760 ([gene|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTCATTTTTATGATTCT,  downstream forward: _UP4_TAGAAAAAGCTCTCCGGGAG
References
12618455,8113162,7565595,29416041,38483038

9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D

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Time of last update: 2024-10-06 03:28:57

Author of last update: Jstuelk