thrC
168
threonine synthase, has a minor threonine dehydratase ([protein|D0CF32BF81AA1DC7BBBC3E9B667A54F97260DF3F|ilvA]) activity
locus
BSU_32250
Molecular weight
37.31 kDa
pI
5.18
function
biosynthesis of threonine
product
threonine synthase
essential
no
ec
4.2.3.1
synonyms
thrC, thrB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0498 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,313,770 3,314,828
Phenotypes of a mutant
auxotrophic for threonine [pubmed|32743959]
The protein
Catalyzed reaction/ biological activity
O-phospho-L-homoserine + H2O --> L-threonine + phosphate (according to UniProt)
has additional threonine dehydratase activity [pubmed|27260660]
Protein family
threonine synthase family (single member, according to UniProt)
[wiki|Cofactors]
PLP
Structure
[PDB|6CGQ] (complexed with PLP and PLP-Ala) [pubmed|30830751]
[PDB|6NMX] (complexed with PLP and (Z)-L-2-amino-5-phosphono-3-pentenoic acid (APPA)) [Pubmed|30830751]
[AF|P04990]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|0F40291BB10DB6D7DFD94B9C7D700F4410C4EE69|yutH]-[gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]-[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]-[gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]
description
[Pubmed|22383849]
regulation
expressed during [wiki|sporulation] [Pubmed|22383849]
genes
[gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]-[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]-[gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]
description
[Pubmed|12107147]
regulation
expressed in the presence of lysine or cysteine ([wiki|ThrR]) [Pubmed|27260660]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|24163341], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|25755103], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]: repression, [Pubmed|27260660], in [regulon|protein:A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR regulon]
Biological materials
Mutant
GP3030 [gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]::''spc'', available in [wiki|Jrg Stlke]'s lab [pubmed|32743959]
1A773 (''thrC''::''cat''), [Pubmed|8973347], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A773&Search=1A773 BGSC] and in [wiki|Jrg Stlke]'s lab
BKE32250 ([gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
BKK32250 ([gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
References
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Time of last update: 2024-12-21 16:55:42
Author of last update: Jstuelk