hom
168
homoserine dehydrogenase (NADPH)
locus
BSU_32260
Molecular weight
47.33 kDa
pI
4.9
function
biosynthesis of methionine and threonine
product
homoserine dehydrogenase (NADPH)
essential
no
ec
1.1.1.3
synonyms
hom
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0460 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,314,828 3,316,129
The protein
Catalyzed reaction/ biological activity
L-aspartate 4-semialdehyde + NAD(P)H+H+ --> L-homoserine + NAD(P)+ [pubmed|6786715]
Protein family
Homoserine dehydrogenase family (single member, according to UniProt)
[wiki|Domains]
[wiki|ACT domain] (aa 350-426) (according to UniProt)
[wiki|Cofactors]
NADPH [pubmed|6786715]
Structure
[PDB|2EJW] (from ''Thermus thermophilus hb8'', 37% identity, 57% similarity)
[AF|P19582]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|0F40291BB10DB6D7DFD94B9C7D700F4410C4EE69|yutH]-[gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]-[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]-[gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]
description
[Pubmed|22383849]
regulation
expressed during [wiki|sporulation] [Pubmed|22383849]
genes
[gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]-[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]-[gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]
description
[Pubmed|12107147]
regulation
expressed in the presence of lysine or cysteine ([wiki|ThrR]) [Pubmed|27260660]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|24163341], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|25755103], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]: repression, [Pubmed|27260660], in [regulon|protein:A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR regulon]
Biological materials
Mutant
BKE32260 ([gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG
BKK32260 ([gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG
lacZ fusion
pBP312 (in [wiki|pAC5]), available in [wiki|Fabian Commichau]'s lab [Pubmed|27260660]
References
Page visits: 4984
Time of last update: 2024-12-21 20:20:12
Author of last update: Jstuelk