thrR
168
transcription repressor of threonine biosynthetic genes
locus
BSU_27910
Molecular weight
16.52 kDa
pI
6.36
function
control of threonine biosynthesis
product
transcription repressor of threonine biosynthetic genes
essential
no
synonyms
thrR, pheB, yszB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4492 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,852,157 2,852,600
Phenotypes of a mutant
resistance to serine [pubmed|32743959]
The protein
Catalyzed reaction/ biological activity
control of the threonine biosynthetic genes (''[gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]-[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]-[gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]'' and ''[gene|548E92526BC00999031D6EDE43FE061CE0448AFC|thrD]'') [Pubmed|27260660]
Protein family
UPF0735 family (single member, according to UniProt)
[wiki|Domains]
putative DNA binding signature (aa 19-51)
[wiki|ACT domain] (aa 70-145) (according to UniProt)
Structure
[AF|P21204]
Effectors of protein activity
lysine and cysteine (indirectly) inhibit DNA-binding activity of ThrR [Pubmed|27260660]
Expression and Regulation
Operons
genes
[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]-[gene|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg]-[gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]-[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2537815], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
expression is repressed by binding of [wiki|RpoE] to the A-rich sequence in the -35 region of the promoter [Pubmed|27679485]
Biological materials
Mutant
BKE27910 (''[gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]''::''erm'', available in the BGSC and in [wiki|Jörg Stülke]'s and [wiki|Fabian Commichau]'s labs) [Pubmed|27260660]
BKE27910 ([gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCATCCCCCCTTTAA, downstream forward: _UP4_GGTGCATAAGGGAGAGAAAA
BKK27910 ([gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCATCCCCCCTTTAA, downstream forward: _UP4_GGTGCATAAGGGAGAGAAAA
Expression vectors
pGP2290: expression of ''thrR'' in B. subtilis (in[wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
pBP323 (N-terminal Strep-tag, in [wiki|pGP172]) (available in [wiki|Fabian Commichau]'s lab) [Pubmed|27260660]
pBP620 (N-terminal Strep-tag, for [wiki|SPINE], purification from ''B. subtilis'', in [wiki|pGP380]) (available in [wiki|Fabian Commichau]'s lab) [Pubmed|27260660]
pBP622 (C-terminal Strep-tag, for [wiki|SPINE], purification from ''B. subtilis'', in [wiki|pGP382]) (available in [wiki|Fabian Commichau]'s lab) [Pubmed|27260660]
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab [Pubmed|27260660]
lacZ fusion
pGP2291 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Fabian Commichau] Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]
References
Page visits: 5813
Time of last update: 2025-04-09 18:20:16
Author of last update: Jstuelk