nrdE
168

ribonucleoside-diphosphate reductase (alpha subunit), control of DNA/cell mass ratio

Locus

BSU_17380

Molecular weight

80.50 kDa

Isoelectric point

5.93

Protein length

700 aaSequenceBlast

Gene length

2103 bpSequenceBlast

Function

synthesis of deoxyribonucleoside triphosphates

Product

ribonucleoside-diphosphate reductase (alpha subunit)

Essential

yes

E.C.

1.17.4.1

Synonyms

nrdE

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0209 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

1,868,969 1,871,071

Phenotypes of a mutant

essential PubMed

The protein

Catalyzed reaction/ biological activity

[thioredoxin]-disulfide + 2'-deoxyribonucleoside 5'-diphosphate + H₂O --> [thioredoxin]-dithiol + ribonucleoside 5'-diphosphate (according to UniProt)

required for stationary-phase mutagenesis PubMed

Protein family

ribonucleoside diphosphate reductase large chain family (with NrdEB, according to UniProt)

Structure

6CGL (PDB) (pH 4, dAMP bound) PubMed

6CGM (PDB) (pH 7, apo-protein) PubMed

6CGN (PDB) (pH 7, dAMP bound) PubMed

6MT9 (PDB) (TTP in substrate specificity site, ADP in inhibitory site (M-site), and ATP in activity site (I-site)) PubMed

6MV9 (PDB) (TTP in substrate specificity site, ADP in inhibitory site (M-site)) PubMed

6MVE (PDB) (TTP in S-site, ADP in M-site, ATP in I-site, and C-terminal cysteine residues captured in active site) PubMed

6MW3 (PDB) (cyro-EM structure of inhibitory filament formed with NrdF PubMed

6MYX (PDB) (cryo-EM structure of inhibitory filament) PubMed

P50620 (AlphaFold)

Effectors of protein activity

ATP stimulates CDP and UDP reduction PubMed

dATP at concentrations up to ~10 M stimulate CDP and UDP reduction, while higher concentrations inhibit this activity, inhibition by dATP is enhanced by binding of dAMP to the N-terminal domain of NrdE PubMed

TTP stimulates GDP reduction PubMed

dGTP stimulates ADP reduction PubMed

Kinetic information

Steady-state kinetics PubMed

Paralogous protein(s)

NrdEB

Expression and Regulation

Operons

Genes

nrdI-nrdE-nrdF-ymaB

Description

PubMed