trpA
168
tryptophan synthase (alpha subunit)
locus
BSU_22630
Molecular weight
29.30 kDa
pI
4.82
function
biosynthesis of tryptophan
product
tryptophan synthase (alpha subunit)
essential
no
ec
4.2.1.20
synonyms
trpA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0159 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,371,508 2,372,311
The protein
Catalyzed reaction/ biological activity
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine --> D-glyceraldehyde 3-phosphate + H2O + L-tryptophan (according to UniProt)
Protein family
TrpA family (single member, according to UniProt)
Structure
[PDB|1WQ5] (from ''Escherichia coli'', 35% identity, 53% similarity) [Pubmed|15667212]
[AF|P07601]
Expression and Regulation
Operons
genes
[gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]-[gene|4CD2A930753175B7F533EA6B91478577CE80A29C|trpD]-[gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]-[gene|C6872585F9BD3717A4F49E96F7684C4A5AD1760D|trpF]-[gene|AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE|trpB]-[gene|155FE26C148F7022948DD7539533AB6876571862|trpA]
description
[Pubmed|21815947]
regulation
not expressed if tryptophan is available ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]) [Pubmed|1551827]
the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
the [wiki|RNA switch] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
[protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]: transcription termination, [pubmed|1551827], in [regulon|protein:E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB regulon]
genes
[gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]-[gene|4CD2A930753175B7F533EA6B91478577CE80A29C|trpD]-[gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]-[gene|C6872585F9BD3717A4F49E96F7684C4A5AD1760D|trpF]-[gene|AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE|trpB]-[gene|155FE26C148F7022948DD7539533AB6876571862|trpA]-[gene|4D7D62B4CDA350DCDC04E6934B5E23ECE4C57870|hisC]-[gene|DEB8358CE34F80560BE91F036659EF2C67A19623|tyrA]-[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]
description
[Pubmed|21815947]
regulation
not expressed if tryptophan is available ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]) [Pubmed|1551827]
the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
regulatory mechanism
[protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]: transcription termination, [pubmed|1551827], in [regulon|protein:E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB regulon]
Biological materials
Mutant
BKE22630 ([gene|155FE26C148F7022948DD7539533AB6876571862|trpA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTTCTGATGGTTGAAGAT, downstream forward: _UP4_TACAGTTTAAAATGAGGTGA
BKK22630 ([gene|155FE26C148F7022948DD7539533AB6876571862|trpA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTTCTGATGGTTGAAGAT, downstream forward: _UP4_TACAGTTTAAAATGAGGTGA
References
Reviews
Original Publications
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Time of last update: 2025-04-17 15:34:10
Author of last update: Jstuelk