trpA

trpA
168

tryptophan synthase (alpha subunit)

Locus
BSU_22630
Molecular weight
29.30 kDa
Isoelectric point
4.82
Protein length
267 aaSequenceBlast
Gene length
804 bpSequenceBlast
Function
biosynthesis of tryptophan
Product
tryptophan synthase (alpha subunit)
Essential
no
E.C.
4.2.1.20
Synonyms
trpA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0159 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,371,508  2,372,311
The protein
Catalyzed reaction/ biological activity
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine --> D-glyceraldehyde 3-phosphate + H2O + L-tryptophan (according to UniProt)
Protein family
TrpA family (single member, according to UniProt)
Structure
1WQ5 (PDB) (from Escherichia coli, 35% identity, 53% similarity) PubMed
P07601 (AlphaFold)
Expression and Regulation
Operons
Genes
trpE-trpD-trpC-trpF-trpB-trpA
Description
PubMed
Regulation
not expressed if tryptophan is available (MtrB) PubMed
the mRNA is substantially stabilized upon depletion of RNase Y PubMed
the RNA switch RNA is degraded by RNase Y PubMed
Regulatory mechanism
MtrB: transcription termination, PubMed, in mtrB regulon
Open in new tab

trpE→trpA

2025-04-15 07:19:19

Jstuelk

88

53eef6e8739e58d1bed57b78e584c86a2e6ff379

37D11B58C2266E362D5C193ADEA68D53F64A90E3

Genes
trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE
Description
PubMed
Regulation
not expressed if tryptophan is available (MtrB) PubMed
the mRNA is substantially stabilized upon depletion of RNase Y PubMed
Regulatory mechanism
MtrB: transcription termination, PubMed, in mtrB regulon
Open in new tab

trpE→aroE

2025-04-15 07:16:53

Jstuelk

120

a3b37416002f0f1e65847bd8d15ba18b53400752

AA88BDB7192FA5467301E36C102EEEBB21BEDA86

Biological materials
Mutant
BKE22630 (trpA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTTCTGATGGTTGAAGAT,  downstream forward: _UP4_TACAGTTTAAAATGAGGTGA
BKK22630 (trpA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTTCTGATGGTTGAAGAT,  downstream forward: _UP4_TACAGTTTAAAATGAGGTGA
References
Reviews
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Original Publications
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155FE26C148F7022948DD7539533AB6876571862

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Time of last update: 2025-04-27 17:07:14

Author of last update: Jstuelk