trpE
168
anthranilate synthase (subunit I)
locus
BSU_22680
Molecular weight
57.95 kDa
pI
5.25
function
biosynthesis of tryptophan
product
anthranilate synthase (subunit I)
essential
no
ec
4.1.3.27
synonyms
trpE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0147 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,375,869 2,377,416
The protein
Catalyzed reaction/ biological activity
chorismate + L-glutamine --> anthranilate + H+ + L-glutamate + pyruvate (according to UniProt)
Protein family
Anthranilate synthase component I family (with [protein|B189AD3E3D18876E1956763182D835A151727392|pabB], according to UniProt)
Structure
[PDB|1I7Q] (from ''Serratia marcescens'', 42% identity, 62% similarity) [Pubmed|11371633]
[AF|P03963]
Effectors of protein activity
subject to feedback inhibtion by tryptophan [Pubmed|4956345]
Expression and Regulation
Operons
genes
[gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]-[gene|4CD2A930753175B7F533EA6B91478577CE80A29C|trpD]-[gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]-[gene|C6872585F9BD3717A4F49E96F7684C4A5AD1760D|trpF]-[gene|AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE|trpB]-[gene|155FE26C148F7022948DD7539533AB6876571862|trpA]
description
[Pubmed|21815947]
regulation
not expressed if tryptophan is available ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]) [Pubmed|1551827]
the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
the [wiki|RNA switch] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
[protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]: transcription termination, [pubmed|1551827], in [regulon|protein:E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB regulon]
genes
[gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]-[gene|4CD2A930753175B7F533EA6B91478577CE80A29C|trpD]-[gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]-[gene|C6872585F9BD3717A4F49E96F7684C4A5AD1760D|trpF]-[gene|AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE|trpB]-[gene|155FE26C148F7022948DD7539533AB6876571862|trpA]-[gene|4D7D62B4CDA350DCDC04E6934B5E23ECE4C57870|hisC]-[gene|DEB8358CE34F80560BE91F036659EF2C67A19623|tyrA]-[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]
description
[Pubmed|21815947]
regulation
not expressed if tryptophan is available ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]) [Pubmed|1551827]
the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
regulatory mechanism
[protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]: transcription termination, [pubmed|1551827], in [regulon|protein:E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB regulon]
Biological materials
Mutant
BKE22680 ([gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTCTCACTCCTTATG, downstream forward: _UP4_GCTGATGAACAGATTTCTAC
BKK22680 ([gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTCTCACTCCTTATG, downstream forward: _UP4_GCTGATGAACAGATTTCTAC
References
Reviews
Other original publications
The ''trpE'' [wiki|RNA switch]
Page visits: 5471
Time of last update: 2025-01-29 01:03:19
Author of last update: Melvin.boenninger