trpD
168
anthranilate phosphoribosyltransferase
locus
BSU_22670
Molecular weight
35.87 kDa
pI
4.9
function
biosynthesis of tryptophan
product
anthranilate phosphoribosyltransferase
essential
no
ec
2.4.2.18
synonyms
trpD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0547 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,374,881 2,375,897
The protein
Catalyzed reaction/ biological activity
diphosphate + N-(5-phospho-β-D-ribosyl)anthranilate --> 5-phospho-α-D-ribose 1-diphosphate + anthranilate (according to UniProt)
Protein family
anthranilate phosphoribosyltransferase family (single member, according to UniProt)
Structure
[PDB|4YI7] (from Acinetobacter sp., 42% identity)
[AF|P03947]
Expression and Regulation
Operons
genes
[gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]-[gene|4CD2A930753175B7F533EA6B91478577CE80A29C|trpD]-[gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]-[gene|C6872585F9BD3717A4F49E96F7684C4A5AD1760D|trpF]-[gene|AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE|trpB]-[gene|155FE26C148F7022948DD7539533AB6876571862|trpA]
description
[Pubmed|21815947]
regulation
not expressed if tryptophan is available ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]) [Pubmed|1551827]
the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
the [wiki|RNA switch] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
[protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]: transcription termination, [pubmed|1551827], in [regulon|protein:E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB regulon]
genes
[gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]-[gene|4CD2A930753175B7F533EA6B91478577CE80A29C|trpD]-[gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]-[gene|C6872585F9BD3717A4F49E96F7684C4A5AD1760D|trpF]-[gene|AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE|trpB]-[gene|155FE26C148F7022948DD7539533AB6876571862|trpA]-[gene|4D7D62B4CDA350DCDC04E6934B5E23ECE4C57870|hisC]-[gene|DEB8358CE34F80560BE91F036659EF2C67A19623|tyrA]-[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]
description
[Pubmed|21815947]
regulation
not expressed if tryptophan is available ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]) [Pubmed|1551827]
the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
regulatory mechanism
[protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]: transcription termination, [pubmed|1551827], in [regulon|protein:E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB regulon]
Biological materials
Mutant
BKE22670 ([gene|4CD2A930753175B7F533EA6B91478577CE80A29C|trpD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCACCGGCAGTAAGGGTTT, downstream forward: _UP4_CTAAAGCAGAAAGAGGAAGA
BKK22670 ([gene|4CD2A930753175B7F533EA6B91478577CE80A29C|trpD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTCACCGGCAGTAAGGGTTT, downstream forward: _UP4_CTAAAGCAGAAAGAGGAAGA
References
Reviews
Original Publications
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Time of last update: 2025-01-18 00:59:07
Author of last update: Melvin.boenninger