ktrB

ktrB
168

high affinity potassium channel KtrA-KtrB, integral membrane subunit

Locus
BSU_31100
Molecular weight
48.27 kDa
Isoelectric point
10.12
Protein length
445 aaSequenceBlast
Gene length
1338 bpSequenceBlast
Function
potassium uptake
Product
high affinity potassium channel KtrA-KtrB, integral membrane subunit
Essential
no
Synonyms
ktrB, yubG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0168 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,189,089  3,190,426
Phenotypes of a mutant
a kimA ktrA ktrB mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source PubMed
inactivation of ktrB facilitates the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate PubMed
a kimA ktrA-ktrB double mutant is unable to adapt to the presence of 1.2 M NaCl PubMed
The protein
Catalyzed reaction/ biological activity
uptake of potassium
Protein family
TrkH potassium transport family (with KtrD, according to UniProt)
Structure
4J7C (PDB) (the KtrA-KtrB complex) PubMed
8K1T (PDB) (the KtrA-KtrB complex in ATP-bound state with addition of MgCl2) PubMed
8K1S (PDB) (the KtrA-KtrB complex in ADP-bound state) PubMed
O32081 (AlphaFold)
Kinetic information
the KtrA-KtrB channel has a high affinity for potassium,this is determined by KtrB PubMed
Paralogous protein(s)
KtrD
Expression and Regulation
Operons
Genes
ktrA-ktrB
Description
PubMed
Regulatory mechanism
c-di-AMP Riboswitch: RNA switch, expression is switched off upon binding of c-di-AMP, in c-di-AMP Riboswitch
Open in new tab

ktrA→ktrB

2025-03-27 10:14:50

ghost

133

26a6fe341a783d36d890ffc444805dfdfe1546b2

0D50A42396785200FADF8E4BEF6B3A6058277227

Additional information
growth at extreme potassium limitation results in the acquisition of promoter mutations with increased ktrA-ktrB expression PubMed
Biological materials
Mutant
MGNA-A227 (yubG::erm), available at the NBRP B. subtilis, Japan
1A954 ( ktrB::kan), PubMed, available at BGSC
GHB1 (ktrA-ktrB::aphA3), available in Erhard Bremer's lab
GP92 (ktrA-ktrB::aphA3), available in Jörg Stülke's lab PubMed
GP2083 (ktrA-ktrB::aphA3 ktrC::tet), available in Jörg Stülke's lab PubMed
GP2498 (ktrA-ktrB::spc kimA::cat), available in Jörg Stülke's lab
BKE31100 (ktrB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCTTTGTCTACATCCCTT,  downstream forward: _UP4_TGATATCAAAAAAATCCGGC
BKK31100 (ktrB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCTTTGTCTACATCCCTT,  downstream forward: _UP4_TGATATCAAAAAAATCCGGC
GP2716 (ktrA-ktrB::spc), available in Jörg Stülke's lab PubMed
GP3064 (ktrB::kan), available in Jörg Stülke's lab PubMed
Expression vectors
pGP2992 (N-terminal 6xHis-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
FLAG-tag construct
GP2277 (based on pGP1331), available in Jörg Stülke's lab PubMed
Labs working on this gene/protein
Erhard Bremer, University of Marburg, Germany Homepage
Inga Hänelt, Frankfurt, Germany Homepage
João H Morais-Cabral, University of Porto, Portugal Homepage
Jörg Stülke, University of Göttingen, Germany Homepage
References
Reviews
Loading
Original Publications
Loading

A287B9771A56D03ADCA603C1969CBF03DCB10E95

Page visits: 4993

Time of last update: 2025-04-07 23:37:52

Author of last update: Jstuelk