acuB
168
unknown
locus
BSU_29700
Molecular weight
24.21 kDa
pI
6.59
function
unknown
product
unknown
essential
no
synonyms
acuB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3448 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,040,751 → 3,041,395
The protein
[wiki|Domains]
2 [wiki|CBS domain]s (aa 7-66, aa 78-135) (according to UniProt)
Structure
[PDB|5AWE] (from Thermus thermophilus, 30% identity) [pubmed|26936147]
[AF|P39066]
[wiki|Localization]
Cytoplasm (Homogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA]-[gene|FC9981CFB9D7EB8ACDA3032EC2F0538988F277AA|acuB]-[gene|1255813797A83D835E0656A2AAAD361B5BB2094B|acuC]
description
[Pubmed|7934817]
regulation
repressed by glucose (3.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|7913927,12850135]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|7913927], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7913927], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is quite stable (half-life 5 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
Biological materials
Mutant
BKE29700 (Δ[gene|FC9981CFB9D7EB8ACDA3032EC2F0538988F277AA|acuB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTTTTTCCCCTTTGT, downstream forward: _UP4_CCGTCAGAGCAAAGGGATCT
BKK29700 (Δ[gene|FC9981CFB9D7EB8ACDA3032EC2F0538988F277AA|acuB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTTTTTCCCCTTTGT, downstream forward: _UP4_CCGTCAGAGCAAAGGGATCT
Expression vectors
pGP2922 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-11-09 01:18:26
Author of last update: Melvin.boenninger