acuA
168
protein acetylase for the control of [protein|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA] and [protein|16412BAB04BBF9FD1C3653328923687874C81868|menE] activity
locus
BSU_29690
Molecular weight
24.18 kDa
pI
5.03
function
control of [protein|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA] and [protein|16412BAB04BBF9FD1C3653328923687874C81868|menE] activity
product
Gcn5-related N-acetyltransferase
essential
no
synonyms
acuA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
3,040,092 3,040,724
The protein
Catalyzed reaction/ biological activity
acetylates (and thereby inactivates) [protein|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA] on Lys-549 and [protein|16412BAB04BBF9FD1C3653328923687874C81868|menE] on Lys-471 [Pubmed|18487328,19136592,39422507]
Protein family
[wiki|Acetyltransferase family] (according to UniProt)
Gcn5-relatedN-acetyltransferase [Pubmed|18487328]
[wiki|Domains]
[wiki|N-acetyltransferase domain] (aa 20-161) (according to UniProt)
Structure
[PDB|2Q04] (from Exiguobacterium sibiricum, 59% identity)
[AF|P39065]
Expression and Regulation
Operons
genes
[gene|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA]-[gene|FC9981CFB9D7EB8ACDA3032EC2F0538988F277AA|acuB]-[gene|1255813797A83D835E0656A2AAAD361B5BB2094B|acuC]
description
[Pubmed|7934817]
regulation
repressed by glucose (3.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|7913927,12850135]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|7913927], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7913927], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is quite stable (half-life 5 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
Biological materials
Mutant
GP1211 (''acuA''::''kan''), available in [wiki|Jörg Stülke]'s lab
GP1255 (''[gene|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA]''::''kan'' ''[gene|6072A6EF0766F3D1B1616A20CDC36CCDC6CBDA71|yjcK]''::''cat'' ''[gene|18FA5EFCD78C74296F9E52A64DA4A9D635944528|ynaD]''::''spec'' ''[gene|4A8360E50ADBA4CC859A64A72EBC0A07F70A1764|yoaA]''::''tet''), available in [wiki|Jörg Stülke]'s lab
BKE29690 ([gene|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTAATTCACCGTCCCATC, downstream forward: _UP4_TAACTGACACTGACAAAGGG
BKK29690 ([gene|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTAATTCACCGTCCCATC, downstream forward: _UP4_TAACTGACACTGACAAAGGG
References
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Time of last update: 2024-12-21 20:46:29
Author of last update: Jstuelk