adaA
168
trigger enzyme
locus
BSU_01810
Molecular weight
24.15 kDa
pI
9.19
function
adaptive response to alkylative DNA damage
product
trigger enzyme
essential
no
synonyms
adaA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2169 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
203,729 204,364
Phenotypes of a mutant
enhanced formation of [gene|F1969E4C7BAAF70BCBE570F58350C12A3E417539|rpsB] or [gene|144D952B05EBBFF41EC92DF906543EA00475FE69|rpsE] suppressor mutants after mitomycin treatment [pubmed|34339280]
The protein
Catalyzed reaction/ biological activity
methylphosphotriester deoxyribonucleoside in DNA + L-cysteinyl-[protein] --> deoxyribonucleotide in DNA + H+ + S-methyl-L-cysteinyl-[protein] (according to UniProt)
Protein family
[wiki|AraC family]
[wiki|Domains]
[wiki|HTH araC/xylS-type domain] (aa 102-200) (according to UniProt)
Structure
[PDB|1ZGW] (from E. coli, 39% identity) [pubmed|16209950]
[AF|P19219]
Expression and Regulation
Operons
genes
[gene|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]-[gene|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB]
description
[Pubmed|2120677]
regulation
positive control by [protein|search|AdaA] [Pubmed|2120677]
regulatory mechanism
[protein|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]: activation, in [regulon|protein:0F8A564256A598DB3A36668FA0C984542FE1323F|adaA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2120677], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE01810 ([gene|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATCTCACCTCTCCTT, downstream forward: _UP4_ATGAGTAAAATGGAGGAAAC
BKK01810 ([gene|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATCTCACCTCTCCTT, downstream forward: _UP4_ATGAGTAAAATGGAGGAAAC
References
Reviews
Original Publications
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Time of last update: 2024-12-21 19:06:21
Author of last update: Jstuelk