adaB
168
O6-methylguanine-DNA methyltransferase
locus
BSU_01820
Molecular weight
19.98 kDa
pI
6.15
function
adaptive response to alkylative DNA damage
product
O6-methylguanine-DNA methyltransferase
essential
no
ec
2.1.1.63
synonyms
adaB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0350 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
204,351 → 204,890
The protein
Catalyzed reaction/ biological activity
6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] --> 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)
4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] --> thymidine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)
Protein family
MGMT family (with [protein|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt], according to UniProt)
Structure
[PDB|4BHB] (from Mycobacterium tuberculosis, 42% identity) [pubmed|23564173]
[AF|P19220]
Expression and Regulation
Operons
genes
[gene|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]-[gene|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB]
description
[Pubmed|2120677]
regulation
positive control by [protein|search|AdaA] [Pubmed|2120677]
regulatory mechanism
[protein|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]: activation, in [regulon|protein:0F8A564256A598DB3A36668FA0C984542FE1323F|adaA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2120677], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE01820 (Δ[gene|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAGTAAAGACCAATAAAGGG, downstream forward: _UP4_TGAAAGTGAATCATCTATAA
BKK01820 (Δ[gene|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAGTAAAGACCAATAAAGGG, downstream forward: _UP4_TGAAAGTGAATCATCTATAA
References
Reviews
Original Publications
Page visits: 2516
Time of last update: 2025-04-09 20:53:00
Author of last update: Melvin.boenninger