metQ

metQ
168

methionine ABC transporter (binding lipoprotein)

Locus
BSU_32730
Molecular weight
30.20 kDa
Isoelectric point
8.49
Protein length
274 aaSequenceBlast
Gene length
825 bpSequenceBlast
Function
methionine uptake
Product
methionine ABC transporter (binding lipoprotein)
Essential
no
Synonyms
metQ, yusA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1464 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,361,767  3,362,591
The protein
Catalyzed reaction/ biological activity
binding of external methionine
Protein family
nlpA lipoprotein family (with YhcJ, according to UniProt)
Structure
4GOT (PDB)
O32167 (AlphaFold)
Paralogous protein(s)
YhcJ
Localization
associated to the cell membrane, via MetP PubMed
extracellular (signal peptide) PubMed,  lipid modification as retention signal
Expression and Regulation
Operons
Genes
metN-metP-metQ
Description
PubMed
Regulation
activated during growth in the presence of branched chain amino acids (CodY) PubMed
the mRNA is processed between metP and metQ by RNase Y, this requires the RicA-RicF-RicT complex PubMed
the S-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
S-box: RNA switch, the S-box riboswitch binds S-adenosylmethionine resulting in termination, in S-box
CodY: activation, PubMed, in codY regulon
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metN→metQ

2025-04-11 08:15:47

Jstuelk

86

9ad5323c9fe97f5a93af04eabd407c1ff456e082

2838D0C991A12719802237E588CE3A803C3DF6A2

Biological materials
Mutant
MGNA-A595 (yusA::erm), available at the NBRP B. subtilis, Japan
BKE32730 (metQ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAGCTTTTTCAATGTAAATC,  downstream forward: _UP4_TAAGACTGAAACCCCGGATG
BKK32730 (metQ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAGCTTTTTCAATGTAAATC,  downstream forward: _UP4_TAAGACTGAAACCCCGGATG
References
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Time of last update: 2025-04-15 04:27:07

Author of last update: Jstuelk