SubtInteract
Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.
Now online: A description of SubtiWiki, SubtiPathways, and SubtInteract in the 2012 Database issue of Nucleic Acids Research
Ulrike Mäder, Arne G Schmeisky, Lope A Flórez, Jörg Stülke
SubtiWiki--a comprehensive community resource for the model organism Bacillus subtilis.
Nucleic Acids Res: 2012, 40(Database issue);D1278-87
[PubMed:22096228]
[WorldCat.org]
[DOI]
(I p)
Contents
- 1 Methods to detect protein-protein interactions
- 2 Visualization of protein-protein interactions in B. subtilis
- 3 Protein complexes in B. subtilis
- 3.1 Complexes in Cellular processes
- 3.2 Complexes in metabolism, ATP synthesis and respiration
- 3.3 Complexes in Information processing
- 3.3.1 Initiation of DNA replication: the primosome
- 3.3.2 DNA replication: the replisome
- 3.3.3 origin segregation: condensin
- 3.3.4 transcription: RNA polymerase
- 3.3.5 translation: the ribosome
- 3.3.6 synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln
- 3.3.7 RNA processing and degradation: the RNA degradosome
- 3.3.8 protein secretion: the signal recognition particle
- 3.4 Complexes involved in Lifestyles
- 4 Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)
- 5 Important publications
Methods to detect protein-protein interactions
- Yeast Two Hybrid System PubMed
- TAP-Tag purification PubMed
Attention: Each technique detects only about 33% of all interactions PubMed
Visualization of protein-protein interactions in B. subtilis
- interactive protein-protein interaction map
- the beta version of SubtInteract
Protein complexes in B. subtilis
Complexes in Cellular processes
cell wall synthesis and cell shape: the elongasome
cell division: the divisome
Complexes in metabolism, ATP synthesis and respiration
the metabolons of glycolysis and the TCA cycle PubMed
the ATP synthase
respiratory complexes and supercomplexes
Complexes in Information processing
Initiation of DNA replication: the primosome
DNA replication: the replisome
origin segregation: condensin
transcription: RNA polymerase
translation: the ribosome
synthesis of glutamyl-tRNA(Gln): the transamidosome (GatA-GatB-GatC)-GltX-trnS-Gln
RNA processing and degradation: the RNA degradosome
protein secretion: the signal recognition particle
Complexes involved in Lifestyles
general stress response: the stressosome
DNA uptake: the pseudopilus PubMed
spore germination: the germinosome
Suspected hub proteins potentially involved in a large number of interactions (as deduced from a Yeast two-hybrid analysis)
- FruA, SwrC, XhlA, YhaP, YhgE, YkcC, PgpH, CsbC, CssS, FliZ, MreD, PpsC, RacA, Smc, YclI, YdeL, YdgH, YdbI, RhgR, YkoT, YopZ, YqbD, RmgR, YueB, YwqJ, YyxA
Important publications
Ariel Fernández
Withdrawn: Supramolecular Evolution of Protein Organization.
Annu Rev Genet: 2013;
[PubMed:23808343]
[WorldCat.org]
[DOI]
(I a)
Led Yered Jafet García Montes de Oca, Alicia Chagolla-López, Luis González de la Vara, Tecilli Cabellos-Avelar, Carlos Gómez-Lojero, Emma Berta Gutiérrez Cirlos
The composition of the Bacillus subtilis aerobic respiratory chain supercomplexes.
J Bioenerg Biomembr: 2012, 44(4);473-86
[PubMed:22790590]
[WorldCat.org]
[DOI]
(I p)
Agnès Vendeville, Damien Larivière, Eric Fourmentin
An inventory of the bacterial macromolecular components and their spatial organization.
FEMS Microbiol Rev: 2011, 35(2);395-414
[PubMed:20969605]
[WorldCat.org]
[DOI]
(I p)
Mike P Williamson, Michael J Sutcliffe
Protein-protein interactions.
Biochem Soc Trans: 2010, 38(4);875-8
[PubMed:20658969]
[WorldCat.org]
[DOI]
(I p)
Patrick Amar, Guillaume Legent, Michel Thellier, Camille Ripoll, Gilles Bernot, Thomas Nystrom, Milton H Saier, Vic Norris
A stochastic automaton shows how enzyme assemblies may contribute to metabolic efficiency.
BMC Syst Biol: 2008, 2;27
[PubMed:18366733]
[WorldCat.org]
[DOI]
(I e)
Adam Brymora, Valentina A Valova, Phillip J Robinson
Protein-protein interactions identified by pull-down experiments and mass spectrometry.
Curr Protoc Cell Biol: 2004, Chapter 17;Unit 17.5
[PubMed:18228443]
[WorldCat.org]
[DOI]
(I p)
Hongtao Guan, Endre Kiss-Toth
Advanced technologies for studies on protein interactomes.
Adv Biochem Eng Biotechnol: 2008, 110;1-24
[PubMed:18219467]
[WorldCat.org]
[DOI]
(I p)
Vincent Collura, Guillaume Boissy
From protein-protein complexes to interactomics.
Subcell Biochem: 2007, 43;135-83
[PubMed:17953394]
[WorldCat.org]
[DOI]
(P p)
Philippe Noirot, Marie-Françoise Noirot-Gros
Protein interaction networks in bacteria.
Curr Opin Microbiol: 2004, 7(5);505-12
[PubMed:15451506]
[WorldCat.org]
[DOI]
(P p)
Barry Causier
Studying the interactome with the yeast two-hybrid system and mass spectrometry.
Mass Spectrom Rev: 2004, 23(5);350-67
[PubMed:15264234]
[WorldCat.org]
[DOI]
(P p)
Andrzej Dziembowski, Bertrand Séraphin
Recent developments in the analysis of protein complexes.
FEBS Lett: 2004, 556(1-3);1-6
[PubMed:14706816]
[WorldCat.org]
[DOI]
(P p)
Alfonso Valencia, Florencio Pazos
Computational methods for the prediction of protein interactions.
Curr Opin Struct Biol: 2002, 12(3);368-73
[PubMed:12127457]
[WorldCat.org]
[DOI]
(P p)
Peter Uetz
Two-hybrid arrays.
Curr Opin Chem Biol: 2002, 6(1);57-62
[PubMed:11827824]
[WorldCat.org]
[DOI]
(P p)
P Legrain, J Wojcik, J M Gauthier
Protein--protein interaction maps: a lead towards cellular functions.
Trends Genet: 2001, 17(6);346-52
[PubMed:11377797]
[WorldCat.org]
[DOI]
(P p)
C L Tucker, J F Gera, P Uetz
Towards an understanding of complex protein networks.
Trends Cell Biol: 2001, 11(3);102-6
[PubMed:11306254]
[WorldCat.org]
[DOI]
(P p)
P Uetz, R E Hughes
Systematic and large-scale two-hybrid screens.
Curr Opin Microbiol: 2000, 3(3);303-8
[PubMed:10851163]
[WorldCat.org]
[DOI]
(P p)
Z Chen, M Han
Building a protein interaction map: research in the post-genome era.
Bioessays: 2000, 22(6);503-6
[PubMed:10842303]
[WorldCat.org]
[DOI]
(P p)
P A Srere
Macromolecular interactions: tracing the roots.
Trends Biochem Sci: 2000, 25(3);150-3
[PubMed:10694888]
[WorldCat.org]
[DOI]
(P p)
T Dandekar, B Snel, M Huynen, P Bork
Conservation of gene order: a fingerprint of proteins that physically interact.
Trends Biochem Sci: 1998, 23(9);324-8
[PubMed:9787636]
[WorldCat.org]
[DOI]
(P p)
P A Srere, J Ovadi
Enzyme-enzyme interactions and their metabolic role.
FEBS Lett: 1990, 268(2);360-4
[PubMed:2200717]
[WorldCat.org]
[DOI]
(P p)
P A Srere
Complexes of sequential metabolic enzymes.
Annu Rev Biochem: 1987, 56;89-124
[PubMed:2441660]
[WorldCat.org]
[DOI]
(P p)