gatB

168

production of glutamyl-tRNA(Gln)

Locus: BSU_06690

Molecular weight: 53.24 kDa

Isoelectric point: 4.9

Protein length: 476 aa

Gene length: 1431 bp

Function: translation

Product: glutamyl-tRNA(Gln) amidotransferase (subunit B)

Essential: yes

E.C.: 6.3.5.7

Synonyms: gatB, yerN

Genomic Context

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List of homologs in different organisms, belongs to COG0064 (Galperin et al., 2021)

Gene

Coordinates: 730,509 → 731,939

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-glutamine + L-glutamyl-tRNA^Gln --> ADP + H⁺ + L-glutamate + L-glutaminyl-tRNA^Gln + phosphate (according to UniProt)
• ATP + H₂O + L-aspartyl-tRNA^Asn + L-glutamine --> ADP + 2 H⁺ + L-asparaginyl-tRNA^Asn + L-glutamate + phosphate (according to UniProt)

Protein family

• GatB/GatE family (single member, according to UniProt)

Structure

• 2DF4 (PDB) (GatA-GatB-GatC complex from Staphylococcus aureus) PubMed
• O30509 (AlphaFold)

Expression and Regulation

Operons

• Genes: gatC-gatA-gatB

  Description: PubMed

Biological materials

Mutant

• MGNA-B447 (yerN::erm), available at the NBRP B. subtilis, Japan

References

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