ydeL

168

similar to transcriptional regulator (MocR/ GabR family)

Locus: BSU_05240

Molecular weight: 53.36 kDa

Isoelectric point: 9.22

Protein length: 463 aa

Gene length: 1392 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ydeL

Genomic Context

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List of homologs in different organisms, belongs to COG1167 (Galperin et al., 2021)

Gene

Coordinates: 571,389  572,780

The protein

Protein family

• MocR/ GabR family PubMed
• class-I pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Domains

• N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.
• HTH gntR-type domain (aa 13-81) (according to UniProt)

Cofactors

• PLP

Structure

• 4MGR (PDB) (GabR, 38% identity) PubMed
• P96669 (AlphaFold)

Localization

• nucleoid (Homogeneous) PubMed

Biological materials

Mutant

• MGNA-C083 (ydeL::erm), available at the NBRP B. subtilis, Japan
• BKE05240 (ydeL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCGCGTTGTTCTCCT,  downstream forward: _UP4_TGAGGGAGCTTTCAGTTTAG
• BKK05240 (ydeL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCGCGTTGTTCTCCT,  downstream forward: _UP4_TGAGGGAGCTTTCAGTTTAG

Expression vectors

• pGP3937 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab

References

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