BioI

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Gene name bioI
Synonyms
Essential no
Product cytochrome P450 enzyme
Function biosynthesis of biotin
Gene expression levels in SubtiExpress: bioI
Interactions involving this protein in SubtInteract: BioI
Metabolic function and regulation of this protein in SubtiPathways:
Biotin
MW, pI 44 kDa, 6.2
Gene length, protein length 1185 bp, 395 aa
Immediate neighbours ytbQ, bioB
Sequences Protein DNA DNA_with_flanks
Genetic context
BioI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
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Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

  • Locus tag: BSU30190

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: formation of pimelic acid via a multiple-step oxidative cleavage of long-chain fatty acids
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed in the presence of biotin (BirA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews: PubMed

Original publications

Max J Cryle
Selectivity in a barren landscape: the P450(BioI)-ACP complex.
Biochem Soc Trans: 2010, 38(4);934-9
[PubMed:20658980] [WorldCat.org] [DOI] (I p)

Max J Cryle, Ilme Schlichting
Structural insights from a P450 Carrier Protein complex reveal how specificity is achieved in the P450(BioI) ACP complex.
Proc Natl Acad Sci U S A: 2008, 105(41);15696-701
[PubMed:18838690] [WorldCat.org] [DOI] (I p)

Rachel J Lawson, David Leys, Michael J Sutcliffe, Carol A Kemp, Myles R Cheesman, Susan J Smith, John Clarkson, W Ewen Smith, Ihtshamul Haq, John B Perkins, Andrew W Munro
Thermodynamic and biophysical characterization of cytochrome P450 BioI from Bacillus subtilis.
Biochemistry: 2004, 43(39);12410-26
[PubMed:15449931] [WorldCat.org] [DOI] (P p)

Rachel J Lawson, Claes von Wachenfeldt, Ihtshamul Haq, John Perkins, Andrew W Munro
Expression and characterization of the two flavodoxin proteins of Bacillus subtilis, YkuN and YkuP: biophysical properties and interactions with cytochrome P450 BioI.
Biochemistry: 2004, 43(39);12390-409
[PubMed:15449930] [WorldCat.org] [DOI] (P p)

Max J Cryle, James J De Voss
Carbon-carbon bond cleavage by cytochrome p450(BioI)(CYP107H1).
Chem Commun (Camb): 2004, (1);86-7
[PubMed:14737344] [WorldCat.org] [DOI] (P p)

Max J Cryle, Nick J Matovic, James J De Voss
Products of cytochrome P450(BioI) (CYP107H1)-catalyzed oxidation of fatty acids.
Org Lett: 2003, 5(18);3341-4
[PubMed:12943422] [WorldCat.org] [DOI] (P p)

Amanda J Green, Andrew W Munro, Myles R Cheesman, Graeme A Reid, Claes von Wachenfeldt, Stephen K Chapman
Expression, purification and characterisation of a Bacillus subtilis ferredoxin: a potential electron transfer donor to cytochrome P450 BioI.
J Inorg Biochem: 2003, 93(1-2);92-9
[PubMed:12538057] [WorldCat.org] [DOI] (P p)

A J Green, S L Rivers, M Cheeseman, G A Reid, L G Quaroni, I D Macdonald, S K Chapman, A W Munro
Expression, purification and characterization of cytochrome P450 Biol: a novel P450 involved in biotin synthesis in Bacillus subtilis.
J Biol Inorg Chem: 2001, 6(5-6);523-33
[PubMed:11472016] [WorldCat.org] [DOI] (P p)

J E Stok, J De Voss
Expression, purification, and characterization of BioI: a carbon-carbon bond cleaving cytochrome P450 involved in biotin biosynthesis in Bacillus subtilis.
Arch Biochem Biophys: 2000, 384(2);351-60
[PubMed:11368323] [WorldCat.org] [DOI] (P p)

J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842] [WorldCat.org] [DOI] (P p)

S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940] [WorldCat.org] [DOI] (P p)