bioF

168

8-amino-7-oxononanoate synthase

Locus: BSU_30220

Molecular weight: 42.42 kDa

Isoelectric point: 6.28

Protein length: 389 aa

Gene length: 1170 bp

Function: biosynthesis of biotin

Product: 8-amino-7-oxononanoate synthase

Essential: no

E.C.: 2.3.1.47

Synonyms: bioF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0156 (Galperin et al., 2021)

Gene

Coordinates: 3,092,184  3,093,353

The protein

Catalyzed reaction/ biological activity

• pimeloyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO₂ PubMed

Protein family

• class-II pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 3A2B (PDB) (Serine palmitoyltransferase from Sphingobacterium multivorum, 37% identity) PubMed
• P53556 (AlphaFold)

Paralogous protein(s)

• Kbl

Expression and Regulation

Operons

• Genes: bioW-bioA-bioF-bioD-bioB-bioI-ytbQ

  Description: PubMed

  Regulation

  • repressed in the presence of biotin (BirA) PubMed

  Regulatory mechanism

  • BirA: repression, PubMed, in birA regulon

Biological materials

Mutant

• GP4750 (trpC2 ΔbioF::neo), available in Jörg Stülke's lab
• BKE30220 (bioF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTCTAACCGCTCGTTTAACC,  downstream forward: _UP4_ATCGGAAAGGAGCTGCACAT
• BKK30220 (bioF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTCTAACCGCTCGTTTAACC,  downstream forward: _UP4_ATCGGAAAGGAGCTGCACAT

References

Reviews

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Original Publications

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