SsbA

• Description: single-strand DNA-binding protein, part of the replisome
  
  

Gene name	ssbA
Synonyms	ssb
Essential	yes PubMed
Product	single-strand DNA-binding protein
Function	DNA replication, DNA repair/ recombination
Gene expression levels in SubtiExpress: ssbA
Interactions involving this protein in SubtInteract: SsbA
MW, pI	18 kDa, 4.822
Gene length, protein length	516 bp, 172 aa
Immediate neighbours	rpsR, rpsF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, DNA repair/ recombination, essential genes, phosphoproteins

This gene is a member of the following regulons

ComK regulon, stringent response

The gene

Basic information

• Locus tag: BSU40900

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • SsbA inhibits ssDNA phosphorylase activity of PnpA PubMed
  • SsbA co-assembles onto SsbB-coated ssDNA PubMed

• Protein family:

• Paralogous protein(s): SsbB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-76 PubMed
  • phosphorylated on Tyr-82 by PtkA, dephosphorylated by PtpZ PubMed

• Cofactor(s):

• Effectors of protein activity: Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold PubMed

• Interactions:
  • forms tetramers PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • SsbA-SbcC PubMed
  • SsbA-RecG PubMed
  • SsbA-YrvE PubMed
  • SsbA-YrvN PubMed
  • SsbA (bound to ssDNA) -RecO, this helps to dislodge SsbA and SsbB from ssDNA PubMed
  • SsbA-YqiB PubMed
  • SsbA-Ung PubMed
  • SsbA-YpbB PubMed
  • SsbA-YrrC PubMed
  • DnaE-DnaN PubMed

• Localization:
  • forms foci PubMed
  • Nucleoid (Mid-cell) PubMed

Database entries

• Structure: 3VDY 3VDY (SsbB in complex with ssDNA, 63% identity, 87% similarity) PubMed

• UniProt: P37455

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yyaF-rpsF-ssbA-rpsR PubMed

• Expression browser: ssbA PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Dusica Vujaklija, Boris Macek
Detecting posttranslational modifications of bacterial SSB proteins.
Methods Mol Biol: 2012, 922;205-18
[PubMed:22976189] [WorldCat.org] [DOI] (I p)