comK

168

competence transcription factor (CTF)

Locus: BSU_10420

Molecular weight: 22.28 kDa

Isoelectric point: 7.67

Protein length: 192 aa

Gene length: 579 bp

Function: regulation of genetic competence and DNA uptake

Product: competence transcription factor (CTF)

Essential: no

Synonyms: comK

Genomic Context

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List of homologs in different organisms, belongs to COG4903 (Galperin et al., 2021)

Gene

Coordinates: 1,117,109  1,117,687

Phenotypes of a mutant

• no expression of competence genes, loss of genetic competence PubMed

RNA

Interactions

• as-comK-comK mRNA PubMed

The protein

Structure

• P40396 (AlphaFold)

Modification

• phosphorylated on Arg-65, Arg-157, Arg-161, Arg-165, Arg-186, and Arg-191 PubMed

Expression and Regulation

Operons

• Genes: comK

  Description: PubMed

  Regulation

  • comK has a bistable expression pattern PubMed
  • expression is reduced in a cwlO mutant PubMed

  Regulatory mechanism

  • Rok: repression, PubMed, in rok regulon
  • CodY: repression, PubMed, in codY regulon
  • AbrB: repression, PubMed, in abrB regulon
  • ComK: activation, (autoregulation) PubMed, in comK regulon
  • DegU: activation, in degU regulon
  • Spo0A: activation, Spo0A-P, acts as antagonist of Rok PubMed, in spo0A regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • translation of comK is reduced under stress conditions due to pairing of the mRNA with the SigB-dependent as-comK RNA PubMed

• Genes: comK-yhjA

  Description

  • PubMed

  Regulation

  • as for comK PubMed

  Expression

  • results from read through at the terminator downstream of comK PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Additional information

• full expression of comK requires fully assembled and rotating flagella PubMed
• DegU-P represses comK expression PubMed

Biological materials

Mutant

• 1A871 (no resistance), PubMed, available at BGSC
• BKE10420 (comK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTATGGCCTCCATCCT,  downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT
• BKK10420 (comK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTATGGCCTCCATCCT,  downstream forward: _UP4_TAGAAAAATAGGAAGGAGCT

Expression vectors

• for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

Labs working on this gene/protein

• Oscar Kuipers, University of Groningen, The Netherlands
• Homepage

References

Reviews

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Original Publications

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The ComK regulon

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