EpsH

• Description: extracellular polysaccharide synthesis
  
  

Gene name	epsH
Synonyms	yveR
Essential	no
Product	unknown
Function	extracellular polysaccharide synthesis
MW, pI	39 kDa, 5.788
Gene length, protein length	1032 bp, 344 aa
Immediate neighbours	epsI, epsG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU34300

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: glycosyltransferase 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P71057

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by SinR PubMed

• Regulatory mechanism:
  • SinR: transcription repression PubMed
  • SlrR: transcription activation PubMed

• Additional information:
  • induction by sequestration of SinR by SinI PubMed
  • activation by SlrR requires prior formation of the SlrR-SlrA complex PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

References

Yunrong Chai, Frances Chu, Roberto Kolter, Richard Losick
Bistability and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2008, 67(2);254-63
[PubMed:18047568] [WorldCat.org] [DOI] (P p)

Frances Chu, Daniel B Kearns, Steven S Branda, Roberto Kolter, Richard Losick
Targets of the master regulator of biofilm formation in Bacillus subtilis.
Mol Microbiol: 2006, 59(4);1216-28
[PubMed:16430695] [WorldCat.org] [DOI] (P p)

Daniel B Kearns, Frances Chu, Steven S Branda, Roberto Kolter, Richard Losick
A master regulator for biofilm formation by Bacillus subtilis.
Mol Microbiol: 2005, 55(3);739-49
[PubMed:15661000] [WorldCat.org] [DOI] (P p)

Dacheng Ren, Laura A Bedzyk, Peter Setlow, Stuart M Thomas, Rick W Ye, Thomas K Wood
Gene expression in Bacillus subtilis surface biofilms with and without sporulation and the importance of yveR for biofilm maintenance.
Biotechnol Bioeng: 2004, 86(3);344-64
[PubMed:15083514] [WorldCat.org] [DOI] (P p)

S S Branda, J E González-Pastor, S Ben-Yehuda, R Losick, R Kolter
Fruiting body formation by Bacillus subtilis.
Proc Natl Acad Sci U S A: 2001, 98(20);11621-6
[PubMed:11572999] [WorldCat.org] [DOI] (P p)