epsH

168

undecaprenyl (UnDP) priming UDP-N-acetyl-glucosamine transferase, synthesis of extracellular poly-N-acetylglucosamine

Locus: BSU_34300

Molecular weight: 39.65 kDa

Isoelectric point: 5.79

Protein length: 344 aa

Gene length: 1035 bp

Function: synthesis of extracellular poly-N-acetylglucosamine

Product: undecaprenyl (UnDP) priming UDP-N-acetyl-glucosamine transferase

Essential: no

Synonyms: epsH, yveR

Genomic Context

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List of homologs in different organisms, belongs to COG0463 (Galperin et al., 2021)

Gene

Coordinates: 3,521,111 → 3,522,145

Phenotypes of a mutant

• altered cell death pattern in colonies PubMed

The EAR RNA switch

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The protein

Catalyzed reaction/ biological activity

• production of UnDP-3-O-acyl N- acetylglucosamine PubMed

Protein family

• glycosyltransferase 2 family (according to UniProt)

Structure

• P71057 (AlphaFold)

Paralogous protein(s)

• EpsJ

Expression and Regulation

Operons

• Genes: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO

  Description: PubMed

  Regulation

  • repressed by SinR PubMed

  Regulatory mechanism

  • RemA: activation, PubMed, in remA regulon
  • AbrB: repression, PubMed, in abrB regulon
  • SinR: anti-activation, (prevents binding of RemA) PubMed, in sinR regulon
  • EAR riboswitch: processive antitermination, in EAR riboswitch

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed

Biological materials

Mutant

• MGNA-A067 (yveR::erm), available at the NBRP B. subtilis, Japan
• BKE34300 (ΔepsH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATGACCGGCTCCTCGT,  downstream forward: _UP4_AAAATGAGAAACAGAGGGTG
• BKK34300 (ΔepsH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATGACCGGCTCCTCGT,  downstream forward: _UP4_AAAATGAGAAACAGAGGGTG

Labs working on this gene/protein

• Richard Losick, Harvard Univ., Cambridge, USA homepage

References

Reviews

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