epsC

168

UDP-N-acetylglucosamine 4,6-dehydratase, required for extracellular polysaccharide synthesis, this gene is inactive in B. subtilis 168

Locus: BSU_34350

Molecular weight: 66.09 kDa

Isoelectric point: 8.77

Protein length: 598 aa

Gene length: 1797 bp

Function: Biofilm formation, biosynthesis of UDP-N,N’-diacetylbacillosamine (with EpsN and EpsM)

Product: UDP-N-acetylglucosamine 4,6-dehydratase

Essential: no

Synonyms: epsC, yveM

Genomic Context

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List of homologs in different organisms, belongs to COG1086 (Galperin et al., 2021)

Gene

Coordinates: 3,526,407  3,528,203

The protein

Catalyzed reaction/ biological activity

• UDP-N-acetylglucosamine (UDP-GlcNAc) --> UDP-2,6-dideoxy-2-acetamido 4-keto glucose (UDP-4-keto) PubMed

Protein family

• Polysaccharide synthase family (according to UniProt)

Cofactors

• NAD⁺ PubMed

Structure

• 5BJW (PDB) (from Campylobacter jejuni, corresponds to the C-terminal part of EpsC, aa 242 ... 571, 47% identity) PubMed
• P71052 (AlphaFold)

Paralogous protein(s)

• YodU, (for SpsM)
  YpqP, (for SpsM)

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO

  Description: PubMed

  Regulation

  • repressed by SinR PubMed

  Regulatory mechanism

  • RemA: activation, PubMed, in remA regulon
  • AbrB: repression, PubMed, in abrB regulon
  • SinR: anti-activation, (prevents binding of RemA) PubMed, in sinR regulon
  • EAR riboswitch: processive antitermination, in EAR riboswitch

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed

Biological materials

Mutant

• MGNA-A071 (yveM::erm), available at the NBRP B. subtilis, Japan
• BKE34350 (epsC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGACAGTCTTCTCCGGT,  downstream forward: _UP4_AGCGTTCATTAGGGGGAGTG
• BKK34350 (epsC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGACAGTCTTCTCCGGT,  downstream forward: _UP4_AGCGTTCATTAGGGGGAGTG

References

Reviews

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Research papers

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The EAR RNA switch

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Labs working on this gene/protein

• Richard Losick, Harvard Univ., Cambridge, USA homepage