epsE

168

inhibitor of motility and glycosyltransferase required for EPS biosynthesis

Locus: BSU_34330

Molecular weight: 32.05 kDa

Isoelectric point: 9.8

Protein length: 278 aa

Gene length: 837 bp

Function: biofilm formation

Product: glycosyltransferase, inhibitor of motility

Essential: no

Synonyms: epsE, yveO

Genomic Context

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List of homologs in different organisms, belongs to COG0463 (Galperin et al., 2021)

Gene

Coordinates: 3,524,417  3,525,253

Phenotypes of a mutant

• smooth colonies on MsGG medium, no biofilm formation PubMed

The protein

Catalyzed reaction/ biological activity

• arrests flagellar rotation in a manner similar to that of a clutch, by disengaging motor force-generating elements in cells embedded in the biofilm matrix, separates the cytoplasmic FliG motor from the MotA-MotB stator PubMed
• biosynthesis of extracellular polysaccharides PubMed

Protein family

• glycosyltransferase 2 family (according to UniProt)

Structure

• P71054 (AlphaFold)

Modification

• phosphorylated by EpsB on a Tyr residue PubMed

Localization

• cell membrane, forms spots at flagellar basal bodies PubMed

Expression and Regulation

Operons

• Genes: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO

  Description: PubMed

  Regulation

  • repressed by SinR PubMed

  Regulatory mechanism

  • RemA: activation, PubMed, in remA regulon
  • AbrB: repression, PubMed, in abrB regulon
  • SinR: anti-activation, (prevents binding of RemA) PubMed, in sinR regulon
  • EAR riboswitch: processive antitermination, in EAR riboswitch

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed

Biological materials

Mutant

• MGNA-B613 (yveO::erm), available at the NBRP B. subtilis, Japan
• BKE34330 (epsE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTCATACGCTTTTCTCCTT,  downstream forward: _UP4_AAGCATGAATAGCAGCCAAA
• BKK34330 (epsE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTCATACGCTTTTCTCCTT,  downstream forward: _UP4_AAGCATGAATAGCAGCCAAA

Labs working on this gene/protein

• Daniel Kearns, Indiana University, Bloomington, USA, homepage
• Richard Losick, Harvard Univ., Cambridge, USA homepage

References

Reviews

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Research papers

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The EAR RNA switch

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