SpoIIIAG

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  • Description: component of the SpoIIIA-SpoIIQ type III secretion system residing in the forespore membrane, required for SigG activation

Gene name spoIIIAG
Synonyms
Essential no
Product component of a type III secretion system
Function activation of SigG
MW, pI 25 kDa, 6.407
Gene length, protein length 687 bp, 229 aa
Immediate neighbours spoIIIAH, spoIIIAF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SpoIIIAG context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU24370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: membrane protein PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information: the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Amy H Camp, Richard Losick
A novel pathway of intercellular signalling in Bacillus subtilis involves a protein with similarity to a component of type III secretion channels.
Mol Microbiol: 2008, 69(2);402-17
[PubMed:18485064] [WorldCat.org] [DOI] (I p)

Chris Guillot, Charles P Moran
Essential internal promoter in the spoIIIA locus of Bacillus subtilis.
J Bacteriol: 2007, 189(20);7181-9
[PubMed:17693505] [WorldCat.org] [DOI] (P p)

N Illing, J Errington
The spoIIIA operon of Bacillus subtilis defines a new temporal class of mother-cell-specific sporulation genes under the control of the sigma E form of RNA polymerase.
Mol Microbiol: 1991, 5(8);1927-40
[PubMed:1766372] [WorldCat.org] [DOI] (P p)