spoIIQ

168

component of the SpoIIIAH-SpoIIQ type III secretion system residing in the forespore membrane, required for anchoring of proteins on both sides of the sporulation septum, required for localization and stability of SpoIIE

Locus: BSU_36550

Molecular weight: 30.96 kDa

Isoelectric point: 4.47

Protein length: 283 aa

Gene length: 852 bp

Function: forespore encasement by the spore coat

Product: part of the transmembrane channel linking the mother cell and the forespore

Essential: no

Synonyms: spoIIQ, ywnI

Genomic Context

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List of homologs in different organisms, belongs to COG5820 (Galperin et al., 2021)

Gene

Coordinates: 3,759,702  3,760,553

The protein

Catalyzed reaction/ biological activity

• required for forespore encasement by the spore coat PubMed
• required for the recruitment of SpoIIIAH to the sporulation septum on the mother cell side PubMed
• required for the proper localization and stability of SpoIIE PubMed

Structure

• 3UZO (PDB); 3TUF (PDB) (the SpoIIQ-SpoIIIAH pore forming complex)  PubMed
• P71044 (AlphaFold)

Localization

• membrane protein, forms a transmembrane channel linking the mother cell and the forespore (with SpoIIIAH) PubMed
• septal membranes on the forespore side PubMed
• proper localization requires SpoIIIAH, GerM and degradation of septal peptidoglycan by the cell wall hydrolases SpoIID and SpoIIP PubMed

Expression and Regulation

Operons

• Genes: spoIIQ

  Description: PubMed

  Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed

  Sigma factors

  • SigF: sigma factor, PubMed, in sigF regulon

Biological materials

Mutant

• BKE36550 (spoIIQ::erm trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTTTCATCACCTCAGC,  downstream forward: _UP4_TAATGAAGAAAACGTCTATC
• BKK36550 (spoIIQ::kan trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTTTCATCACCTCAGC,  downstream forward: _UP4_TAATGAAGAAAACGTCTATC

References

Reviews

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Original Publications

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