spoIIID

168

transcriptional regulator (repressor or activator) of a subset of Sigma E-dependent genes

Locus: BSU_36420

Molecular weight: 10.66 kDa

Isoelectric point: 9.5

Protein length: 93 aa

Gene length: 282 bp

Function: regulation of mother cell gene expression

Product: transcriptional regulator

Essential: no

Synonyms: spoIIID

Genomic Context

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Categories containing this gene/protein

Information processing, Regulation of gene expression, Transcription factors and their control, Transcription factors/ other

Lifestyles, Sporulation, Sporulation proteins, Sporulation proteins/ other

List of homologs in different organisms, belongs to COG5830 (Galperin et al., 2021)

This gene is a member of the following regulons

SigE regulon, SpoIIID regulon

Gene

Coordinates: 3,748,421  3,748,702

The protein

Catalyzed reaction/ biological activity

binds its DNA targets as a monomeric protein (possible due to the two DNA-binding motifs) PubMed

Domains

two DNA-binding regions: HTH at the N-terminus and a basic region near the C-terminus PubMed
HTH deoR-type domain (aa 4-75) (according to UniProt)

Structure

2L0K (PDB) (complex with DNA)
P15281 (AlphaFold)

Expression and Regulation

Operons

Genes: usd-spoIIID-mbl

Description: PubMed

Regulation

expressed early during sporulation in the mother cell (SigE) PubMed.

Sigma factors

SigE: sigma factor, PubMed, in sigE regulon

Additional information

An antisense RNA is predicted for 'mbl' PubMed

Biological materials

Mutant

BKE36420 (spoIIID::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCGTGCACACCACTCGACC,  downstream forward: _UP4_TAATCACCTGGCATTGCCTT
BKK36420 (spoIIID::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCGTGCACACCACTCGACC,  downstream forward: _UP4_TAATCACCTGGCATTGCCTT

References

Reviews

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Original Publications

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