spoIIIAG

168

component of the SpoIIIA-SpoIIQ type III secretion system residing in the forespore membrane, required for SigG activation

Locus: BSU_24370

Molecular weight: 25.34 kDa

Isoelectric point: 6.41

Protein length: 229 aa

Gene length: 690 bp

Function: activation of SigG

Product: component of a type III secretion system

Essential: no

Synonyms: spoIIIAG

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5828 (Galperin et al., 2021)

Gene

Coordinates: 2,533,010  2,533,699

The protein

Domains

• contains a ring-building motif (RBM) domain PubMed

Structure

• 5WC3 (PDB) PubMed
• P49784 (AlphaFold)

Localization

• membrane protein, in membranes that surround the forespore, this requires SpoIIIAH and SpoIIQ PubMed
• forms an oligomeric ring-like conduit in the space between the mother cell and forespore membranes PubMed

Expression and Regulation

Operons

• Genes: yqhV-spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH

  Description: PubMed

  Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: spoIIIAG-spoIIIAH

  Description: PubMed

  Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

  Additional information

  • for a discussion of the relevance of the operon-internal promoter, see PubMed

• Genes: spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH

  Description: PubMed

  Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

Biological materials

Mutant

• BKE24370 (spoIIIAG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTATTCATTGCCGACACT,  downstream forward: _UP4_AAAAAAATAAAGGAGGATTC
• BKK24370 (spoIIIAG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTATTCATTGCCGACACT,  downstream forward: _UP4_AAAAAAATAAAGGAGGATTC

References

Reviews

Loading

Original Publications

Loading