AcoL

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  • Description: acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)

Gene name acoL
Synonyms yfjH
Essential no
Product acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)
Function acetoin utilization
Gene expression levels in SubtiExpress: acoL
Interactions involving this protein in SubtInteract: AcoL
Metabolic function and regulation of this protein in SubtiPathways:
acoL
MW, pI 48 kDa, 5.273
Gene length, protein length 1374 bp, 458 aa
Immediate neighbours acoC, acoR
Sequences Protein DNA DNA_with_flanks
Genetic context
AcoL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AcoL expression.png















Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AcoR regulon, CcpA regulon, SigL regulon

The gene

Basic information

  • Locus tag: BSU08090

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein N(6)-(dihydrolipoyl)lysine + NAD+ = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)
  • Protein family: class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:
    • the mRNA is very stable (half-life > 15 min) PubMed
    • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 233 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 555 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

Your additional remarks

References