lpdV

168

2-oxoisovalerate dehydrogenase (E3 subunit, dihydrolipoamide dehydrogenase)

Locus: BSU_24060

Molecular weight: 48.51 kDa

Isoelectric point: 4.89

Protein length: 474 aa

Gene length: 1425 bp

Function: utilization of branched-chain keto acids

Product: 2-oxoisovalerate dehydrogenase (E3 subunit, dihydrolipoamide dehydrogenase)

Essential: no

E.C.: 1.8.1.4

Synonyms: lpdV, yqiV, bkd

Genomic Context

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List of homologs in different organisms, belongs to COG1249 (Galperin et al., 2021)

Gene

Coordinates: 2,500,104  2,501,528

Phenotypes of a mutant

• a strain lacking the ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB operon and the des gene exhibits strongly reduced membrane fluidity and eventual growth arrest PubMed
• defective in Swarming motility (prolonged period of immotility prior to Swarming) PubMed

The protein

Catalyzed reaction/ biological activity

• (R)-N⁶-dihydrolipoyl-L-lysyl-[protein] + NAD⁺ --> (R)-N⁶-lipoyl-L-lysyl-[protein] + H⁺ + NADH (according to UniProt)

Protein family

• class-I pyridine nucleotide-disulfide oxidoreductase family (with PdhD and AcoL, according to UniProt)

Cofactors

• FAD (according to UniProt)

Structure

• 2YQU (PDB) (from Thermus thermophilus (hb8 mutant), 42% identity, 55% similarity)
• P54533 (AlphaFold)

Paralogous protein(s)

• AcoL, PdhD

Expression and Regulation

Operons

• Genes: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB

  Description: PubMed

  Regulation

  • induced in the presence of isoleucine or valine (BkdR) PubMed

  Regulatory mechanism

  • BkdR: activation, PubMed, in bkdR regulon
  • CodY: repression, PubMed, in codY regulon

  Sigma factors

  • SigL: sigma factor, PubMed, in sigL regulon

Biological materials

Mutant

• MGNA-C379 (yqiV::erm), available at the NBRP B. subtilis, Japan
• BKE24060 (lpdV::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTGACTCACTCCTGTC,  downstream forward: _UP4_TAAAAGCATAAAGGAGGGGC
• BKK24060 (lpdV::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTGACTCACTCCTGTC,  downstream forward: _UP4_TAAAAGCATAAAGGAGGGGC

References

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