narJ

168

chaperone for the nitrate reductase (protein J)

Locus: BSU_37260

Molecular weight: 21.06 kDa

Isoelectric point: 4.61

Protein length: 184 aa

Gene length: 555 bp

Function: nitrate respiration, nitrogen assimilation

Product: chaperone for the nitrate reductase (protein J)

Essential: no

E.C.: null

Synonyms: narJ

Genomic Context

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List of homologs in different organisms, belongs to COG2180 (Galperin et al., 2021)

Gene

Coordinates: 3,824,226  3,824,780

The protein

Protein family

• NarJ/NarW family (single member, according to UniProt)

Structure

• P42178 (AlphaFold)

Expression and Regulation

Operons

• Genes: narG-narH-narJ-narI

  Description: PubMed

  Regulation

  • expressed under anaerobic conditions (Fnr) PubMed

  Regulatory mechanism

  • Fnr: activation, PubMed, in fnr regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE37260 (narJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTGTTACACCTCATTT,  downstream forward: _UP4_AGCGTTGAAGGGGCTGTTCA
• BKK37260 (narJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTGTTACACCTCATTT,  downstream forward: _UP4_AGCGTTGAAGGGGCTGTTCA

References

Reviews

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Original Publications

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