Difference between revisions of "EpsC"
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| − | * '''Description:''' | + | * '''Description:''' UDP-sugar epimerase, required for extracellular polysaccharide synthesis, this gene is inactive in ''B. subtilis'' 168 <br/><br/> |
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* '''Protein family:''' polysaccharide synthase family (according to Swiss-Prot) | * '''Protein family:''' polysaccharide synthase family (according to Swiss-Prot) | ||
| − | * '''Paralogous protein(s):''' | + | * '''Paralogous protein(s):''' SpsM ([[YodU]], [[YpqP]]) |
=== Extended information on the protein === | === Extended information on the protein === | ||
Revision as of 08:12, 10 October 2014
- Description: UDP-sugar epimerase, required for extracellular polysaccharide synthesis, this gene is inactive in B. subtilis 168
| Gene name | epsC |
| Synonyms | yveM |
| Essential | no |
| Product | unknown |
| Function | biofilm formation |
| Gene expression levels in SubtiExpress: epsC | |
| Regulation of this protein in SubtiPathways: epsC | |
| MW, pI | 66 kDa, 8.775 |
| Gene length, protein length | 1794 bp, 598 aa |
| Immediate neighbours | epsD, epsB |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biofilm formation, membrane proteins
This gene is a member of the following regulons
AbrB regulon, EAR riboswitch, RemA regulon, SinR regulon
The gene
Basic information
- Locus tag: BSU34350
Phenotypes of a mutant
Database entries
- BsubCyc: BSU34350
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- Correction of sfp, epsC, swrAA, and degQ as well as introduction of rapP from a plasmid present in NCIB3610 results in biofilm formation in B. subtilis 168 PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: polysaccharide synthase family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU34350
- Structure:
- UniProt: P71052
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
- induction by sequestration of SinR by SinI or SlrA PubMed
- the EAR riboswitch (eps-associated RNA switch) located between epsB and epsC mediates processive antitermination and allows expression of the long eps operon PubMed
- the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO operon is not expressed in a ymdB mutant PubMed
- the amount of the mRNA is substantially decreased upon depletion of RNase Y (this is likely due to the increased stability of the sinR mRNA) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Richard Losick, Harvard Univ., Cambridge, USA homepage
Your additional remarks
References
Reviews
Original publications
The EAR RNA switch
Regulation of the eps operon
Other original publications
Anna L McLoon, Sarah B Guttenplan, Daniel B Kearns, Roberto Kolter, Richard Losick
Tracing the domestication of a biofilm-forming bacterium.
J Bacteriol: 2011, 193(8);2027-34
[PubMed:21278284]
[WorldCat.org]
[DOI]
(I p)
